Nitric acid-induced bronchiolitis in rats mimics childhood bronchiolitis obliterans

Nitric acid-induced bronchiolitis in rats mimics childhood bronchiolitis obliterans

Autor Costa, CLB Google Scholar
Spilborghs, GMGT Google Scholar
Martins, M. A. Google Scholar
Saldiva, PHN Google Scholar
Mauad, T. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Background: Childhood bronchiolitis obliterans (CBO) is an infrequent but severe disease, characterized by persistent obstructive respiratory symptoms after an acute episode of bronchiolitis. Its etiology is mostly postviral, especially in developing countries. Pathologically, CBO is characterized as constrictive-type BO. the pathogenesis of this disease is poorly understood and there is no specific treatment. Animal models could help to better understand the sequence of events culminating in bronchiolar obliteration and serve as basis for experimental therapeutic research. Objectives: in this study, we adapted the nitric acid (NA) technique to develop a suitable model to study CBO in rats. Methods: We have intratracheally instilled 0.5% NA or saline and studied the histological lesions on days 2, 7, 14 and 30. NA-instilled animals developed a sequence of bronchiolar lesions that included necrotizing bronchiolitis, proliferative (PROL) bronchiolar lesions and constricitive (CONS) bronchiolar lesions with bronchiectasis, mucostasis and macrophagic accumulation. Results: the frequency of PROL lesions decreased and that of the CONS lesions increased, with a statistically significant difference at the sacrifice days, except for day 7 (PROL vs. CONS lesions day 2: p = 0.001; PROL vs. CONS lesions day 7: p = 0.38; PROL vs. CONS lesions day 14: p < 0.001; PROL vs. CONS lesions day 30: p < 0.001). Conclusions: We conclude that this model presents histological similarities with the histological picture of CBO, being suitable for further developmental and therapeutic experimental research in this field. Copyright (C) 2005 S. Karger AG, Basel.
Palavra-chave bronchiolitis obliterans
animal model
rats
pathology
nitric acid
children
Idioma Inglês
Data de publicação 2005-01-01
Publicado em Respiration. Basel: Karger, v. 72, n. 6, p. 642-649, 2005.
ISSN 0025-7931 (Sherpa/Romeo, fator de impacto)
Publicador Karger
Extensão 642-649
Fonte http://dx.doi.org/10.1159/000087363
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000233952900015
Endereço permanente http://repositorio.unifesp.br/handle/11600/28107

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