Functional interface between cathepsins and growth factors in the kidney development

Functional interface between cathepsins and growth factors in the kidney development

Autor Vattimo, Maria de Fatima Fernandes Google Scholar
Santos, Oscar Fernando Pavao Autor UNIFESP Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo During kidney development many proteases are involved with the remodeling process of the extracellular matrix (ECM) during nephrogenesis. This study used embryonic kidneys culture, tridimensional cell culture, and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques in order to investigate the expression of cathepsins S (CS) and cathepsin H (CH) during metanephrogenesis and their functional interface with hepatic growth factor (HGF) and nerve growth factor (NGF). Results have shown that cathepsin S has been expressed early than the cathepsin H in the nephrogenesis. NGF antibody in the embryonic kidney cultures, in a dose-dependent mechanism inhibited the CS but not CH genic expression by RT-PCR. the tridimensional cells culture with MDCK and IMCD cells confirmed the interface between HGF and CS and CH once their inhibitors added to the culture, reduced the fancy branching formation induced by this growth factor. in summary, this study suggests that CS and CH are differently expressed during nephrogenesis and also that they are involved with the tubulogenesis probably mediating specific growth factors such as NGF and HGF.
Palavra-chave growth factors
cathepsins
metanephrogenesis
extracellular matrix
Idioma Inglês
Data de publicação 2005-01-01
Publicado em Renal Failure. Philadelphia: Taylor & Francis Inc, v. 27, n. 5, p. 615-622, 2005.
ISSN 0886-022X (Sherpa/Romeo, fator de impacto)
Publicador Taylor & Francis Inc
Extensão 615-622
Fonte http://dx.doi.org/10.1080/08860220500200486
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000231674500019
Endereço permanente http://repositorio.unifesp.br/handle/11600/28096

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