Virulence of Paracoccidioides brasiliensis and gp43 expression in isolates bearing known PbGP43 genotype

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dc.contributor.author Carvalho, K. C.
dc.contributor.author Ganiko, L.
dc.contributor.author Batista, W. L.
dc.contributor.author Morais, F. V.
dc.contributor.author Marques, E. R.
dc.contributor.author Goldman, G. H.
dc.contributor.author Franco, M. E.
dc.contributor.author Puccia, R.
dc.date.accessioned 2016-01-24T12:37:34Z
dc.date.available 2016-01-24T12:37:34Z
dc.date.issued 2005-01-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2004.09.008
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 7, n. 1, p. 55-65, 2005.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28084
dc.description.abstract Paracoccidioides brasiliensis is the dimorphic fungus responsible for human paracoccidioidomycosis (PCM). We previously observed that P. brasiliensis isolates bearing highly polymorphic PbGP43 of genotype A (Pb2, Pb3 and Pb4) were phylogenetically distant from the others. the PbGP43 gene encodes an immune dominant diagnostic antigen (gp43), and its polymorphism reflects broader genetic diversity in the species. in the present study, we observed that isolates with PbGP43 of genotype A showed low virulence when inoculated in B10.A mice by the intraperitoneal, intratracheal and intravenous routes. in vitro studies detected sharp and prolonged down-regulation of PbGP43 in Pb3 (and not in PbI8 or Pb339) as a result of heat shock at 42degreesC and temperature shift to prompt mycelium to yeast transition, which was, however, not disturbed. Differences in transcriptional regulation are possibly a consequence of mutations in the PbGP43 promoter region, which we here show to be more polymorphic in genotype A isolates. As opposed to Pb3's rapid adaptation to in vitro culture conditions after isolation from the lung, Pb12, the most aggressive isolate tested here, showed slow growth and phase transition in vitro. Interestingly, animals that were highly infected by Pb12 produced small amounts of anti-gp43 antibodies. That was apparently due to down-regulation in PbGP43 expression. We present the first evidence of transcriptional regulation of gp43 expression, but our results suggest that gene expression is also regulated at the protein and/or secretion levels. (C) 2004 Elsevier SAS. All rights reserved. en
dc.format.extent 55-65
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso restrito
dc.subject gp43 en
dc.subject virulence en
dc.subject Paracoccidioides brasiliensis en
dc.title Virulence of Paracoccidioides brasiliensis and gp43 expression in isolates bearing known PbGP43 genotype en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation UNIFESP, Disciplina Biol Celular, Dept Microbiol Imunol & Parasitol, BR-0423062 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Patol, São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Disciplina Biol Celular, Dept Microbiol Imunol & Parasitol, BR-0423062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Patol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.micinf.2004.09.008
dc.description.source Web of Science
dc.identifier.wos WOS:000227029400007



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