Frequency of V alpha 24(+)V beta 11(+) NKT cells in peripheral blood of human kidney transplantation recipients

Frequency of V alpha 24(+)V beta 11(+) NKT cells in peripheral blood of human kidney transplantation recipients

Autor Galante, N. Z. Google Scholar
Ozaki, K. S. Google Scholar
Cenedeze, M. A. Google Scholar
Kallas, E. G. Google Scholar
Salomão, Reinaldo Autor UNIFESP Google Scholar
Pacheco-Silva, A. Google Scholar
Camara, NOS Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo There are still lacking data supporting a role for natural killer T (NKT) cells in the maintenance of human tissue-specific tolerance. We are interested to study NKT cell frequency in kidney transplant recipients and its correlation with graft function. Peripheral blood T cell receptors (TCR) Valpha24(+)Vbeta11(+) NKT cells were phenotyped according to CD4 and CD8 expression in normal controls (NC), in 10 years rejection-free cadaver kidney allografts maintained with minimal immunosuppression longterm rejection free [LTRF]), in patients with acute rejection (AR) and in patients with acute tubular necrosis (ATN). Results were expressed as percentages of CD4(+)CD8(-) (CD4(+) NKT) or CD4(-)CD8(-) (double negative-DN NKT) Valpha24(+)Vbeta11(+) cells. the percentages of Valpha24(+)Vbeta11(+) cells were 0.09%, 0.14%, 0.02% and 0.09% on gated lymphocytes respectively in AR, ATN, LTRF and NC groups (p=0.263). DN NKT cells were more frequent in NC patients (52.11%) and less present in ATN patients (11.04%). in contrast, CD4(+) NKT (IL-4-producing NKT cells subset) was more frequent in AR (42.86%). and corresponded to almost 3 to 7 folds more what we obtained in the other groups. Although total Valpha24(+)Vbeta11(+) cells did not significantly differ among the groups, the lowest frequency was observed in the LTRF group. in conclusion. we observed that total number of NKT cells did not differ significantly among transplant patients when compared to normal controls, although specific-subsets seem to be more frequent in determined events. (C) 2004 Elsevier B.V. All rights reserved.
Palavra-chave T lymphocytes
kidney transplantation
Idioma Inglês
Data de publicação 2005-01-01
Publicado em International Immunopharmacology. Amsterdam: Elsevier B.V., v. 5, n. 1, p. 53-58, 2005.
ISSN 1567-5769 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 53-58
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000226615400007
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