Dissemination and diversity of metallo-beta-lactamases in Latin America: report from the SENTRY Antimicrobial Surveillance Program

Dissemination and diversity of metallo-beta-lactamases in Latin America: report from the SENTRY Antimicrobial Surveillance Program

Autor Sader, H. S. Google Scholar
Castanheira, M. Google Scholar
Mendes, R. E. Google Scholar
Toleman, M. Google Scholar
Walsh, T. R. Google Scholar
Jones, R. N. Google Scholar
Instituição JONES Grp
Universidade Federal de São Paulo (UNIFESP)
Univ Bristol
Resumo Carbapenem resistance among Pseudomonas aeruginosa and Acinetobacter spp. is becoming a critical therapeutic problem worldwide. the SENTRY Antimicrobial Surveillance Program monitors pathogen frequency and antimicrobial resistance patterns of nosocomial and community-acquired infections through sentinel hospitals on five continents. Pseudomonas spp. and Acinetobacter Spp. strains resistant to imipenem (MIC, greater than or equal to 16 mg/l), meropenem (MIC, greater than or equal to 16 mg/l), and ceftazidime (MIC. greater than or equal to32 mg/l) collected from January 2001 to December 2003 were routinely screened for antimicrobial resistance genes. Resistant isolates were initially tested for metallo-beta-lactamase (MbetaL) production by phenotypic tests (disk approximation or MbetaL Etest strip) and then characterization of the MbetaL (hydrolysis assays, PCR for bla(IMP). bla(VIM), bla(SPM) gene sequencing). Eighty-nine isolates (33 Acinetobacter spp., 54 Pseudomonas aeruginosa. and 2 P. fluorescens) had positive phenotypic screening tests. Among those, 34 isolates producing MbetaL were identified, including 7 Acinetobacter spp., 25 P aeruginosa and 2 R fluorescens. the MbetaLs identified were IMP-1. VIM-2 and two newly described enzymes: SPM-1 and IMP-16. the greatest concentration of MOL strains was in Brazil, where imipenem-resistant P. aeruginosa increased significantly in the time period evaluated by the SENTRY Program. MbetaL-producing P aeruginosa was detected in São Paulo (SPM-1) and Brasilia (SPM-1 and IMP-16). Brazil and Caracas, Venezuela (VIM-2); while MbetaL-producing Acinetobacter spp. isolates were detected in São Paulo, Brazil (IMP-1). P. fluorescens isolates producing IMP-1 and VIM-2 were detected in São Paulo, Brazil and Santiago, Chile, respectively. the emergence and dissemination of mobile NXIOLproducing isolates represent an alarming factor for increasing resistance to carbapenems in several medical centres evaluated by the SENTRY Program in Latin America. (C) 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Palavra-chave metallo-beta-lactamases
Pseudomonas aeruginosa
Acinetobacter spp. imipenem-resistance nosocomial infection
Latin America
Idioma Inglês
Data de publicação 2005-01-01
Publicado em International Journal of Antimicrobial Agents. Amsterdam: Elsevier B.V., v. 25, n. 1, p. 57-61, 2005.
ISSN 0924-8579 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 57-61
Fonte http://dx.doi.org/10.1016/j.ijantimicag.2004.08.013
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000226479400008
Endereço permanente http://repositorio.unifesp.br/handle/11600/28079

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