Author |
Marquez, A. S.
![]() Vicentini, A. P. ![]() Ono, M. A. ![]() Watanabe, MAE ![]() Camargo, Z. P. de ![]() Itano, E. N. ![]() |
Institution | Universidade Estadual de Londrina (UEL) Adolfo Lutz Inst Universidade Federal de São Paulo (UNIFESP) |
Abstract | Yeast forms of Paracoccidioides brasiliensis produce polydispersed high molecular mass (h-MM) antigens. We investigated the antibodies to an h-MM antigen from P. brasiliensis by immunoblotting and ELISA in sera from paracoccidioidomycosis (PCM) patients. IgG from the sera of chronic PCIVI patients was able to recognize the h-MM antigen at a higher frequency in the cell-free antigen (CFA) (8/13) than in the somatic antigen (SA) (2/13), as assessed by immunoblotting. the CFA was fractionated by Sephadex G-200 chromatography, and fraction 17 (F17) with the h-MM antigen of approximately 366 kDa was used in ELISA to analyze specific levels of IgG and IgE. Patients with the chronic form showed significantly higher levels of IgG (P < 0.05) but not IgE (P > 0.05) to F17 by ELISA, compared to patients with the acute form or to healthy donors. in conclusion, CFA is better than SA as a source of the P. brasiliensis h-MM antigen. This study reveals a new characteristic to differentiate between the acute and chronic forms of PCM, by demonstrating a higher level of seric IgG to h-MM antigen in chronic compared to acute PCM patients. |
Keywords |
Paracoccidioides brasiliensis
paracoccidioidomycosis high molecular mass antigen IgG IgE |
Language | English |
Date | 2005-01-01 |
Published in | Journal of Clinical Laboratory Analysis. Hoboken: Wiley-liss, v. 19, n. 5, p. 199-204, 2005. |
ISSN | 0887-8013 (Sherpa/Romeo, impact factor) |
Publisher | Wiley-Blackwell |
Extent | 199-204 |
Origin |
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Access rights | Closed access |
Type | Article |
Web of Science ID | WOS:000232330900005 |
URI | http://repositorio.unifesp.br/handle/11600/28070 |
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