Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle

Autor Navarrete, Maria Alicia H Autor UNIFESP Google Scholar
Maier, Carolina M. Google Scholar
Falzoni, Roberto Google Scholar
Quadros, Luiz Gerk de Azevedo Autor UNIFESP Google Scholar
Lima, Geraldo R. Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Nazario, Afonso CP Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Stanford Univ
APC Pathol
Resumo Introduction During the menstrual cycle, the mammary gland goes through sequential waves of proliferation and apoptosis. in mammary epithelial cells, hormonal and non-hormonal factors regulate apoptosis. To determine the cyclical effects of gonadal steroids on breast homeostasis, we evaluated the apoptotic index ( AI) determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling ( TUNEL) staining in human mammary epithelial cells during the spontaneous menstrual cycle and correlated it with cellular proliferation as determined by the expression of Ki-67 during the same period.Methods Normal breast tissue samples were obtained from 42 randomly selected patients in the proliferative ( n = 21) and luteal ( n = 21) phases. Menstrual cycle phase characterization was based on the date of the last and subsequent menses, and on progesterone serum levels obtained at the time of biopsy.Results the proliferation index ( PI), defined as the number of Ki-67-positive nuclei per 1,000 epithelial cells, was significantly larger in the luteal phase (30.46) than in the follicular phase (13.45; P = 0.0033). the AI was defined as the number of TUNEL-positive cells per 1,000 epithelial cells. the average AI values in both phases of the menstrual cycle were not statistically significant ( P = 0.21). However, the cell renewal index ( CRI = PI/AI) was significantly higher in the luteal phase ( P = 0.033). A significant cyclical variation of PI, AI and CRI was observed. PI and AI peaks occurred on about the 24th day of the menstrual cycle, whereas the CRI reached higher values on the 28th day.Conclusions We conclude that proliferative activity is dependent mainly on hormonal fluctuations, whereas apoptotic activity is probably regulated by hormonal and non-hormonal factors.
Palavra-chave apoptosis
Ki-67
mammary gland
menstrual cycle
TdT-mediated dUTP nick end labeling
Idioma Inglês
Data de publicação 2005-01-01
Publicado em Breast Cancer Research. London: Biomed Central Ltd, v. 7, n. 3, p. R306-R313, 2005.
ISSN 1465-5411 (Sherpa/Romeo, fator de impacto)
Publicador Biomed Central Ltd
Extensão R306-R313
Fonte http://dx.doi.org/10.1186/bcr994
Direito de acesso Acesso aberto Open Access
Tipo Resenha
Web of Science WOS:000229844100012
Endereço permanente http://repositorio.unifesp.br/handle/11600/28066

Exibir registro completo




Arquivo

Nome: WOS000229844100012.pdf
Tamanho: 579.1KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta