Disruption of cortical development as a consequence of repetitive pilocarpine-induced status epilepticus in rats

Disruption of cortical development as a consequence of repetitive pilocarpine-induced status epilepticus in rats

Autor Silva, Alexandre Valotta da Autor UNIFESP Google Scholar
Regondi, T. C. Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Spreafico, Roberto Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Ist Nazl Neurol Carlo Besta
Resumo Purpose: the aim of the present study was to observe possible cortical abnormalities after repetitive pilocarpine-induced status epilepticus (SE) in rats during development. Methods: Wistar rats received intraperitoneal injection of pilocarpine hydrochloride 2% (380 mg/kg) at P7, P8, and P9. All experimental rats displayed SE after pilocarpine injections. Rats were killed at P10 and P35, and immunocytochemistry procedures were performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament(SMI-311), parvalburnin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Selected sections were used for the TUNEL method and double-labeling experiments, with different mixtures of the same markers.Results: the major findings of the present work were (a) altered intracortical circuitry development; (b) anticipation of PV immunoreactivity in neocortical interneurons; (c) increased GAD-65 immunoreactivity; and (d) reduced neocortical apoptotic process.Conclusions: From these results, we suggest that previously healthy brain, without genetic abnormalities, might develop an acquired disruption of cortical development whose evolution reproduces some characteristics of the childhood epilepsies associated with cognitive impairment. Key Words: Immature brain-Early insult-Apoptosis-Neocortex-Interneurons.
Palavra-chave immature brain
early insult
apoptosis
neocortex
interneurons
Idioma Inglês
Data de publicação 2005-01-01
Publicado em Epilepsia. Oxford: Blackwell Publishing, v. 46, p. 22-30, 2005.
ISSN 0013-9580 (Sherpa/Romeo, fator de impacto)
Publicador Blackwell Publishing
Extensão 22-30
Fonte http://dx.doi.org/10.1111/j.1528-1167.2005.01003.x
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000230499100005
Endereço permanente http://repositorio.unifesp.br/handle/11600/28058

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