Integron carrying a novel metallo-beta-lactamase gene, bla(IMP-16), and a fused form of aminoglycoside-resistant gene aac(6')-30/aac(6')-Ib': Report from the SENTRY antimicrobial surveillance program

Integron carrying a novel metallo-beta-lactamase gene, bla(IMP-16), and a fused form of aminoglycoside-resistant gene aac(6')-30/aac(6')-Ib': Report from the SENTRY antimicrobial surveillance program

Autor Mendes, Rodrigo E. Autor UNIFESP Google Scholar
Toleman, Mark A. Google Scholar
Ribeiro, Julival Google Scholar
Sader, Helio S. Autor UNIFESP Google Scholar
Jones, Ronald N. Google Scholar
Walsh, Timothy R. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Hosp Base Distrito Fed
Univ Bristol
JONES Grp
Tufts Univ
Resumo Since January 2002 Pseudomonas sp. strains resistant to carbapenems and ceftazidime have been routinely screened as part of the SENTRY Antimicrobial Surveillance Program for metallo-beta-lactamase production, and their resistance determinants have been analyzed. Pseudomonas aeruginosa index strain 101-4704, which harbors a novel bla(IMP) variant, bla(IMP-16), was isolated in April 2002 from a 60-year-old man in Brasilia, Brazil. bla(IMP-16) was found on the chromosome of the P. aeruginosa index strain, and the deduced amino acid sequence (IMP-16) showed the greatest identities to IMP-11 (90.3%) and IMP-8 (89.5%). Sequence analysis revealed that blaIMP-16 was associated with a class 1 integron, which also encoded aminoglycoside-modifying enzymes. Downstream of bla(IMP-16) resided an open reading frame, which consisted of a new aminoglycoside-modifying gene, namely, aac(6')-30, which was fused with aac(6')-Ib'. the amino acid sequence of the aac(6')-30 putative protein showed the most identity (52.7%) to the sequence of AAC(6')-29b described previously. the fourth gene cassette constituted aadA1. the steady-state kinetics of IMP-16 demonstrated that the enzyme preferred cephalosporins and carbapenems to penicillins. the main functional difference observed among the kinetic values for IMP-16 compared to those for other IMPs was a lack of cefoxitin hydrolysis and a lower k(cat)/K-m value for imipenem (0.36 muM(-1) (.) s(-1)). This report further emphasizes the spread of metallo-beta-lactamase genes and their close association with various aminoglycoside resistance genes.
Idioma Inglês
Data de publicação 2004-12-01
Publicado em Antimicrobial Agents and Chemotherapy. Washington: Amer Soc Microbiology, v. 48, n. 12, p. 4693-4702, 2004.
ISSN 0066-4804 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 4693-4702
Fonte http://dx.doi.org/10.1128/AAC.48.12.4693-4702.2004
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225474500030
Endereço permanente http://repositorio.unifesp.br/handle/11600/28026

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