Molecular characterization of a beta-lactamase gene, bla(GIM-1), encoding a new subclass of metallo-beta-lactamase

Molecular characterization of a beta-lactamase gene, bla(GIM-1), encoding a new subclass of metallo-beta-lactamase

Autor Castanheira, Mariana Autor UNIFESP Google Scholar
Toleman, M. A. Google Scholar
Jones, R. N. Google Scholar
Schmidt, F. J. Google Scholar
Walsh, T. R. Google Scholar
Instituição Univ Bristol
Universidade Federal de São Paulo (UNIFESP)
Jones Grp
Inst Lab Med
Resumo As part of the SENTRY Antimicrobial Surveillance Program in 2002, five multidrug-resistant Pseudomonas aeruginosa clinical isolates were detected with metallo-beta-lactamase (MbetaL) activity. the isolates were recovered from different patients in a medical center located in Dusseldorf, Germany. the resistant determinant was isolated amplifying the region between the integrase and the aacA4 gene cassette. Sequencing revealed a novel MbetaL gene, designated bla(GIM-1). Additional analysis showed that GIM-1, comprising 250 amino acids and with a pI value of 5.4, differs in its primary sequence from that described for IMP, VIM, and SPM-1 enzymes by 39 to 43%, 28 to 31%, and 28%, respectively. the enzyme possesses unique amino acids within the major consensus sequence (HXHXD) of the MbetaL family. Kinetics analysis revealed that GIM-1 has no clear preference for any substrate and did not hydrolyze azlocillin, aztreonam, and the serine-beta-lactamase inhibitors. bla(GIM-1) was found on a 22-kb nontransferable plasmid. the new MOL gene was embedded in the first position of a 6-kb class 1 integron, In77, with distinct features, including an aacA4 cassette downstream of the MbetaL gene that appeared to be truncated with bla(GIM-1). the aacA4 was followed by an aadA1 gene cassette that was interrupted by a copy of the IS1394. This integron also carried an oxacillinase gene, bla(OXA-2), before the 3'-CS region. GIM-1 appears to be a unique MbetaL, which is located in a distinct integron structure, and represents the fourth subclass of mobile MbetaL enzymes to be characterized.
Idioma Inglês
Data de publicação 2004-12-01
Publicado em Antimicrobial Agents and Chemotherapy. Washington: Amer Soc Microbiology, v. 48, n. 12, p. 4654-4661, 2004.
ISSN 0066-4804 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 4654-4661
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225474500024
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