Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation

Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation

Autor Carvalho, ACP Google Scholar
Sharpe, J. Google Scholar
Rosenstock, T. R. Google Scholar
Teles, AFV Google Scholar
Youle, R. J. Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
NINDS
Universidade de São Paulo (USP)
Resumo In the present study, we evaluated proapoptotic protein Bax on mitochondria and Ca2+ homeostasis in primary cultured astrocytes. We found that recombinant Bax (rBax, 10 and 100 ng/ml) induces a loss in mitochondrial membrane potential (DeltaPsi(m)). This effect might be related to the inhibition of respiratory rates and a partial release of cytochrome c, which may change mitochondrial morphology. the loss of DeltaPsi(m) and a selective permeabilization of mitochondrial membranes contribute to the release of Ca2+ from the mitochondria. This was inhibited by cyclosporin A (5 muM) and Ruthenium Red (1 mug/ml), indicating the involvement of mitochondrial Ca2+ transport mechanisms. Bax-induced mitochondrial Ca2+ release evokes Ca2+ waves and wave propagation between cells. Our results show that Bax induces mitochondrial alteration that affects Ca2+ homeostasis and signaling. These changes show that Ca2+ signals might be correlated with the proapoptotic activities of Bax.
Palavra-chave Bax
calcium signaling
mitochondria
endoplasmic reticulum
calcium waves
wave propagation
apoptosis
Idioma Inglês
Data de publicação 2004-12-01
Publicado em Cell Death and Differentiation. London: Nature Publishing Group, v. 11, n. 12, p. 1265-1276, 2004.
ISSN 1350-9047 (Sherpa/Romeo, fator de impacto)
Publicador Nature Publishing Group
Extensão 1265-1276
Fonte http://dx.doi.org/10.1038/sj.cdd.4401508
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225109800004
Endereço permanente http://repositorio.unifesp.br/handle/11600/28023

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