A anticonvulsive fraction from Scaptocosa raptoria (Araneae : Lycosidae) spider venom

A anticonvulsive fraction from Scaptocosa raptoria (Araneae : Lycosidae) spider venom

Autor Mussi-Ribeiro, A. Google Scholar
Miranda, A. Google Scholar
Gobbo-Netto, L. Google Scholar
Lopes, N. P. Google Scholar
Santos, W. F. dos Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Several spider neurotoxins are known to show highly selective effects on nervous tissues. Intracerebral injection into rats of spider venom from Scaptocosa raptoria, prevents seizures induced by convulsant agents. Injection of phenytoin (390 pmol/200 nl), muscimol (90 pmol/200 nl), baclofen (500 pmol/200 nl) into the substantia nigra (SN) pars reticulata, protected rats from convulsions evoked by unilateral focal injection of bicuculline into the area tempestas by 50, 80, and 100%, respectively. Denatured S. raptoria crude venom (4.6 mug, 2.3 mug, and 920 ng/200 nl), when administered into the SN, prevented seizures elicited by bicuculline in the area tempestas by 100, 100, and 87.5%, respectively. the injection into the SN of 160 ng/200 nl of fraction SrTx1 isolated from S. raptoria venom, reduced the magnitude of seizures. This fraction was rechromatographed affording fractions SrTx1.1, SrTx1.2 and SrTx1.3, and they were administered into the SN at doses of 100, 200, and 400 ng/200 nl respectively. Fraction SrTx1.3 protected 50, 85.7, and 100% of the animals against the seizures elicited by bicuculline injected into the area tempestas. This suggests that S. raptoria venom as well as its SrTx1.3 fraction, might be potential sources of new anticonvulsant drugs. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Assunto spider venom
Scaptocosa raptoria
GABA agonists
anticonvulsant
bicuculline
seizures
Idioma Inglês
Data 2004-11-23
Publicado em Neuroscience Letters. Clare: Elsevier Sci Ireland Ltd, v. 371, n. 2-3, p. 171-175, 2004.
ISSN 0304-3940 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 171-175
Fonte http://dx.doi.org/10.1016/j.neulet.2004.08.064
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000225203800018
URI http://repositorio.unifesp.br/handle/11600/28022

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