Identification and characterization of a novel factor XIIa inhibitor in the hematophagous insect, Triatoma infestans (Hemiptera : Reduviidae)

Identification and characterization of a novel factor XIIa inhibitor in the hematophagous insect, Triatoma infestans (Hemiptera : Reduviidae)

Autor Campos, Ivan Torres Nicolau de Autor UNIFESP Google Scholar
Tanaka-Azevedo, A. M. Google Scholar
Tanaka, Aparecida Sadae Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Resumo Recently, we have cloned several Kazal-type serine protease inhibitors from the midgut of the Triatoma infestans bug. A single gene composed of multi Kazal-type domains, in tandem, encodes these inhibitors. in this work, we describe the purification and characterization of recombinant infestins 3-4 and 4, which are potent factor XIIa inhibitors (K-i = 67 pM and 128 pM, respectively). We also identified the first native factor XIIa inhibitor from a hematophagous insect. the factor XIIa inhibitory activity of infestin 4 demonstrates extremely efficient anticoagulant activity, prolonging activated partial thromboplastin time by approximately 3 times. Our results suggest that infestins perform a very important role in the T. infestans midgut during meal acquisition and digestion by controlling blood coagulation by means of inhibiting thrombin and factor XIIa. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Palavra-chave factor XIIa
serine protease inhibitor
blood coagulation
insect
hematophagous
Triatoma infestans
Idioma Inglês
Data de publicação 2004-11-19
Publicado em Febs Letters. Amsterdam: Elsevier B.V., v. 577, n. 3, p. 512-516, 2004.
ISSN 0014-5793 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 512-516
Fonte http://dx.doi.org/10.1016/j.febslet.2004.10.052
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225368300037
Endereço permanente http://repositorio.unifesp.br/handle/11600/28021

Exibir registro completo




Arquivo

Nome: WOS000225368300037.pdf
Tamanho: 159.4KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta