A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa

A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa

Autor Cruz-Silva, I Google Scholar
Gozzo, A. J. Google Scholar
Nunes, V. A. Google Scholar
Carmona, A. K. Google Scholar
Faljoni-Alario, A. Google Scholar
Oliva, MLV Google Scholar
Sampaio, M. U. Google Scholar
Sampaio, CAM Google Scholar
Araujo, M. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Caesalpinia echinata is a tree belonging to the Leguminosae family. the red color of the trunk, looking like burning wood ('brasa' in Portuguese), is the origin of the name Brazil. Seeds of leguminous plants contain high amounts of serine proteinase inhibitors that can affect different biological processes. Here we show that a protein isolated from seeds of C. echinata is able to inhibit enzymes that participate in blood coagulation and fibrinolysis. This inhibitor (CeKI) was purified to homogeneity by ion exchange and reversed-phase chromatography. SDS-PAGE indicated a single polypeptide chain with a molecular mass of 20 kDa. CeKI inhibits human plasma kallikrein K-i=3.1 nm), plasmin K-i=0.18 nm), factor XIIa (K-i=0.18 nm), trypsin K-i=21.5 nm) and factor Xa K-i=0.49 mm). CeKI inhibited kinin release from high-molecular-mass kininogen by kallikrein in vitro. the N-terminal sequence, determined by automatic Edman degradation, identified the inhibitor as a member of the Kunitz family. the secondary structure, determined by circular dichroism, is mainly a random coil followed by P-sheet structure. the action of CeKI on enzymes of the blood-clotting intrinsic pathway was confirmed by prolongation of the activated partial thromboplastin time.
Palavra-chave amino acid sequence
Caesalpinia echinata
circular dichroism
kinin release
Kunitz-type inhibitor
serine proteinase inhibitor
Idioma Inglês
Data de publicação 2004-11-01
Publicado em Biological Chemistry. Berlin: Walter de Gruyter & Co, v. 385, n. 11, p. 1083-1086, 2004.
ISSN 1431-6730 (Sherpa/Romeo, fator de impacto)
Publicador Walter de Gruyter & Co
Extensão 1083-1086
Fonte http://dx.doi.org/10.1515/BC.2004.140
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000225438200013
Endereço permanente http://repositorio.unifesp.br/handle/11600/28018

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