Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties

Shiga toxin-producing Escherichia coli in healthy young beef steers from Argentina: prevalence and virulence properties

Autor Meichtri, L. Google Scholar
Miliwebsky, E. Google Scholar
Gioffre, A. Google Scholar
Chinen, I Google Scholar
Baschkier, A. Google Scholar
Chillemi, G. Google Scholar
Guth, BEC Google Scholar
Masana, M. O. Google Scholar
Cataldi, A. Google Scholar
Rodriguez, H. R. Google Scholar
Rivas, M. Google Scholar
Instituição Inst Nacl Tecnol Agropecuaria
Minist Salud
Universidade Federal de São Paulo (UNIFESP)
Resumo Between July 1999 and December 2000, the prevalence of Shiga toxin-producing Escherichia coli (STEC) was established in 200 Argentine healthy young beef steers (14-16 months old) grown under local production systems with a feed grain period of 3-4 months, and the STEC strains isolated were examined in regard to their phenotypic and genotypic characteristics.Stool samples (n=70) and rectal swabs (n=130) were taken at the slaughterhouse level.By polymerase chain reaction (PCR), Shiga toxin (stx) gene sequences were detected in 69% of the samples. Eighty-six STEC strains were isolated from 39% of the animals. Serogroups identified, in order of frequency, were: O8 (16 strains), O113 (14), O103 (5), O91 (4), O171 (3), O174 (3), O25 (2), O112 (2), O145 (2), O2, O11, O104, O121, O128, O143, O146, O157. the most frequent serotype isolated was O8:H19 (12.9%). A total of 17 serotypes, including E. coli O157:H7 found in one animal (0.5%), have been previously associated with hemolytic uremic syndrome (HUS), bloody and non-bloody diarrhea in different countries, including Argentina.The prevalent genotype isolated was stx2 (51 of 86, 59.3%). Subtyping of stx2 variants showed the prevalence of stx2vh-b (25.6%) and stx2vh-a types (24.4%), and revealed the presence of an atypical stx2-v. Only 7.0% of STEC strains carried eae, and 33.7% harbored EHEC-hlyA gene. the full virulent genotype (stx/eae/EHEC-hlyA) was found to be present in 4 of the 86 (4.7%) STEC strains isolated.This research indicates that young steers from the main beef-producing area of Argentina are an important reservoir of STEC strains; however, its importance as agents of human diseases in our country has still to be established. (C) 2004 Elsevier B.V. All rights reserved.
Palavra-chave STEC
beef steers
prevalence
virulence
Idioma Inglês
Data de publicação 2004-11-01
Publicado em International Journal of Food Microbiology. Amsterdam: Elsevier B.V., v. 96, n. 2, p. 189-198, 2004.
ISSN 0168-1605 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 189-198
Fonte http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.018
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000224331100008
Endereço permanente http://repositorio.unifesp.br/handle/11600/28005

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