Why do anal wounds heal adequately? A study of the local immunoinflammatory defense mechanisms

Why do anal wounds heal adequately? A study of the local immunoinflammatory defense mechanisms

Autor Paula, P. R. de Google Scholar
Matos, D. Google Scholar
Franco, M. Google Scholar
Speranzini, M. B. Google Scholar
Figueiredo, F. Google Scholar
Santana, ICB de Google Scholar
Chacon-Silva, M. A. Google Scholar
Bassi, D. G. Google Scholar
Instituição Univ Taubate
Universidade Federal de São Paulo (UNIFESP)
Univ Catolica Brasilia
Universidade de Brasília (UnB)
Resumo PURPOSE: the aim of this study was to identify the tissue defense immunoinflammatory mechanisms present in the healing process of anal region wounds resulting from hemorrhoidectomy by the open technique.METHODS: Immunohistochemical techniques were applied to biopsies of anal wounds obtained on Day 0 and Day 6 after surgery from 20 patients with hemorrhoid disease to charactcrize and quantify macrophages, T and B lymphocytes, and natural killer cells in high-power fields (400x). These techniques were also used to identify Cells showing immunoexpression of cytokines (transforming growth factor beta 1, transforming growth factor beta 2, transforming growth factor beta 3) and constitutive and induced nitric oxide synthase. Plasma cells were quantified on slides stained with hernatoxylin and eosin and the presence of immunoglobulin G, immunoglobulin M, and immunoglobulin A secreting cells was investigated by direct immunofluorescence.RESULTS: An acute nonspecific inflammation with no lymphomono-nuclear-plasmacytic component was observed on Day 0. On Day 6, an inflammatory cellular infiltration rich in macrophages and lymphoplasmacytic cells was detected, which documented the participation of innate defense mechanisms and the adaptive tissue response. On Day 6, the mean number of immunoinflammatory cells were as follows: macrophages (CD68+) = 190.3; macrophages (HAM56+) = 184.3; T lymphocytes (CD3+) = 59.6; T lymphocytes (CD45RO+) = 47.7; helper T lymphocytes (CD4+) = 89.2; cytotoxic T lymphocytes (CD8+) = 29.4; B lymphocytes (CD20+) = 64.4; plasma cells = 1.7; natural killer cells (NK1+) = 12.9. Macrophages (HAM56+ and CD68+) were present in significantly higher amounts than those of the remaining ones. B lymphocytes (CD20+) predominated over T lymphocytes (CD3+), although the difference between the two cell types was not significant. Participation of the humoral immune system was characterized by the presence of immunoglobulin G-secreting cells. the cellular immune system was characterized by the identification of T lymphocytes (CD3+ and CD45RO+), most of them belonging to the T helper cell subpopulation (CD4+). These predominated in a significant manner over cytotoxic T lymphocytes (CD8+). Natural killer cells were present in small amounts. There was immunoexpression of constittitive nitric oxide synthase on Day 0 and on Day 6. Induced nitric oxide Synthase was not identified on Day 0 but was present on Day 6. Transforming growth factor beta 2 and transforming growth factor beta 3 were expressed in endothelial cells on Day 0 and on Day 6, and transforming growth factor betal was also expressed in macrophages, endothelial cells, and fibroblasts on Day 6. Transforming growth factor beta I and transforming growth factor beta 2 were expressed significantly in macrophages, whereas transforming growth factor beta 3 Occurred at similar proportions in the three cell types.CONCLUSIONS: the host developed locally innate and immunologic defense adaptive mechanisms. the predominant local defense response involved macrophages. Natural killer cells and immunoexpression of constitutive nitric oxide synthase in endothelial cells were components of the noninduced innate response. in the induced innate response, in addition to neutrophils, there were large numbers of macrophages that were the major cells showing immunoexpression of transforming growth factor beta and induced nitric oxide synthase. the adaptive immunologic response was characterized by T and B lymphocytes. Helper T cells and cytotoxic T cells predominated in the cellular immune response and cytotoxic T cells and natural killer cells were present in small numbers. Secretory immunoglobulin G plasma cells were present in small numbers as a component of the humoral immune system.
Palavra-chave anus
immunology
hemorrhoids
surgery
wound healing
Idioma Inglês
Data de publicação 2004-11-01
Publicado em Diseases of the Colon & Rectum. Philadelphia: Lippincott Williams & Wilkins, v. 47, n. 11, p. 1861-1867, 2004.
ISSN 0012-3706 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão 1861-1867
Fonte http://dx.doi.org/10.1007/s10350-004-0696-7
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000225240300013
Endereço permanente http://repositorio.unifesp.br/handle/11600/27999

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