Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil. V. Cytokine concentrations in experimentally infected Wistar rats

Investigations on the biology, epidemiology, pathology and control of Tunga penetrans in Brazil. V. Cytokine concentrations in experimentally infected Wistar rats

Autor Feldmeier, H. Google Scholar
Witt, L. H. Google Scholar
Schwalfenberg, S. Google Scholar
Ribeiro, R. A. Google Scholar
Cunha, F. Q. Google Scholar
Harms, G. Google Scholar
Mehlhorn, H. Google Scholar
Liesenfeld, O. Google Scholar
Heukelbach, J. Google Scholar
Instituição Charite Univ Med Berlin
Fed Univ Ceara
Universidade Federal de São Paulo (UNIFESP)
Univ Dusseldorf
Mandacaru Fdn
Resumo Tungiasis is caused by the penetration of the female sand. ea Tunga penetrans into the skin of its host. This parasitic skin disease is almost invariably associated with an intense inflammation around embedded fleas, the underlying mechanisms being unknown. A study was undertaken to determine whether Wistar rats can be used as an animal model to assess cytokine kinetics during the natural course of the infection. Laboratory-raised Wistar rats were exposed in cages put on the soil in an area with high human attack rates. Rats were examined daily and blood samples were taken before exposure and at 2, 6, 10, 13, 16 and 20 days after flea penetration. TNF-alpha, IL-1beta, IFN-gamma, IL-4, IL-10 and CINC (a rat cytokine-induced neutrophil chemoattractant and member of the IL-8 family) were determined by enzyme immunoassay. the results showed an increasing serum concentration of TNF-alpha and IL-1beta 10-13 days after penetration and a rapid increase in IL-4 2 days after fleas became embedded. During the natural course of the infection, the ratio of the serum concentration of TNF-a to that of IL-10 decreased, indicating a relative increase in the secretion of the anti-nflammatory cytokine. the treatment of lesions with silicone oil abrogated the natural disease course and changed the pattern of cytokine secretion. We conclude that the Wistar rat is an appropriate model to study immune responses in tungiasis.
Idioma Inglês
Data de publicação 2004-11-01
Publicado em Parasitology Research. New York: Springer, v. 94, n. 5, p. 371-376, 2004.
ISSN 0932-0113 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 371-376
Fonte http://dx.doi.org/10.1007/s00436-004-1219-0
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000224937400010
Endereço permanente http://repositorio.unifesp.br/handle/11600/27998

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