Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta)

Show simple item record

dc.contributor.author Avellar, MCW [UNIFESP]
dc.contributor.author Honda, L. [UNIFESP]
dc.contributor.author Hamil, K. G.[UNIFESP]
dc.contributor.author Yenugu, S. [UNIFESP]
dc.contributor.author Grossman, G.
dc.contributor.author Petrusz, P.
dc.contributor.author French, F. S. [UNIFESP]
dc.contributor.author Hall, S. H. [UNIFESP]
dc.date.accessioned 2016-01-24T12:37:27Z
dc.date.available 2016-01-24T12:37:27Z
dc.date.issued 2004-11-01
dc.identifier http://dx.doi.org/10.1095/biolreprod.104.031740
dc.identifier.citation Biology of Reproduction. Madison: Soc Study Reproduction, v. 71, n. 5, p. 1453-1460, 2004.
dc.identifier.issn 0006-3363
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27991
dc.description.abstract The epididymis protein 2 (EP2) gene, the fusion of two ancestral P-defensin genes, is highly expressed in the epididymis and subject to species-specific regulation at the levels of promoter selection, transcription, and mRNA splicing. EP2 mRNA expression is also androgen dependent, and at least two of the secreted proteins bind spermatozoa. Alternative splicing produces more than 17 different EP2 mRNA variants. in this article, the expression of EP2 variants was profiled in different tissues from the human and rhesus monkey (Macaca mulatta) male reproductive tract using reverse transcriptase-polymerase chain reaction. Different EP2 mRNA variants were identified not only in human and rhesus testis and epididymis but also in the novel sites, seminal vesicle and prostate. Immunolocalization of EP2 protein in epithelial cells from rhesus and human seminal vesicle demonstrated that EP2 transcripts are translated in these tissues. in addition, two novel splicing variants, named EP2R and EP2S, were discovered. EP2C was the only splice variant expressed in all tissues tested from rhesus monkey. However, expression was not detected in human testis or seminal vesicle. for the first time, bactericidal function was demonstrated for EP2C, EP2K, and EP2L. Taken together, the results indicate that EP2 expression is more widespread in the male reproductive tract than realized previously. Whereas the activity of every EP2 variant tested thus far is antibacterial, further investigation may reveal additional physiological roles for EP2 peptides in the primate male reproductive tract. en
dc.format.extent 1453-1460
dc.language.iso eng
dc.publisher Soc Study Reproduction
dc.relation.ispartof Biology of Reproduction
dc.rights Acesso aberto
dc.subject antibacterial en
dc.subject epididymis en
dc.subject epididymis protein 2 (EP2) gene en
dc.subject gene expression en
dc.subject human en
dc.subject male reproductive tract en
dc.subject prostate en
dc.subject rhesus monkey en
dc.subject seminal vesicles en
dc.subject testis en
dc.title Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta) en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ N Carolina
dc.description.affiliation Universidade Federal de São Paulo, Sect Expt Endocrinol, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Pharmacol, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliation Univ N Carolina, Dept Pediat, Chapel Hill, NC 27599 USA
dc.description.affiliation Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Sect Expt Endocrinol, BR-04044020 São Paulo, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Pharmacol, BR-04044020 São Paulo, SP, Brazil
dc.identifier.doi 10.1095/biolreprod.104.031740
dc.description.source Web of Science
dc.identifier.wos WOS:000224713000007



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account