Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta)

Differential expression and antibacterial activity of epididymis protein 2 isoforms in the male reproductive tract of human and rhesus monkey (Macaca mulatta)

Autor Avellar, MCW Autor UNIFESP Google Scholar
Honda, L. Autor UNIFESP Google Scholar
Autor UNIFESP Google Scholar
Yenugu, S. Autor UNIFESP Google Scholar
Grossman, G. Google Scholar
Petrusz, P. Google Scholar
French, F. S. Autor UNIFESP Google Scholar
Hall, S. H. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ N Carolina
Resumo The epididymis protein 2 (EP2) gene, the fusion of two ancestral P-defensin genes, is highly expressed in the epididymis and subject to species-specific regulation at the levels of promoter selection, transcription, and mRNA splicing. EP2 mRNA expression is also androgen dependent, and at least two of the secreted proteins bind spermatozoa. Alternative splicing produces more than 17 different EP2 mRNA variants. in this article, the expression of EP2 variants was profiled in different tissues from the human and rhesus monkey (Macaca mulatta) male reproductive tract using reverse transcriptase-polymerase chain reaction. Different EP2 mRNA variants were identified not only in human and rhesus testis and epididymis but also in the novel sites, seminal vesicle and prostate. Immunolocalization of EP2 protein in epithelial cells from rhesus and human seminal vesicle demonstrated that EP2 transcripts are translated in these tissues. in addition, two novel splicing variants, named EP2R and EP2S, were discovered. EP2C was the only splice variant expressed in all tissues tested from rhesus monkey. However, expression was not detected in human testis or seminal vesicle. for the first time, bactericidal function was demonstrated for EP2C, EP2K, and EP2L. Taken together, the results indicate that EP2 expression is more widespread in the male reproductive tract than realized previously. Whereas the activity of every EP2 variant tested thus far is antibacterial, further investigation may reveal additional physiological roles for EP2 peptides in the primate male reproductive tract.
Palavra-chave antibacterial
epididymis protein 2 (EP2) gene
gene expression
male reproductive tract
rhesus monkey
seminal vesicles
Idioma Inglês
Data de publicação 2004-11-01
Publicado em Biology of Reproduction. Madison: Soc Study Reproduction, v. 71, n. 5, p. 1453-1460, 2004.
ISSN 0006-3363 (Sherpa/Romeo, fator de impacto)
Publicador Soc Study Reproduction
Extensão 1453-1460
Fonte http://dx.doi.org/10.1095/biolreprod.104.031740
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000224713000007
Endereço permanente http://repositorio.unifesp.br/handle/11600/27991

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