Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis

Therapeutic activity of a killer peptide against experimental paracoccidioidomycosis

Autor Travassos, Luiz R Autor UNIFESP Google Scholar
Silva, Luis. S. Autor UNIFESP Google Scholar
Rodrigues, Elaine G. Autor UNIFESP Google Scholar
Conti, S. Google Scholar
Salati, A. Google Scholar
Magliani, W. Google Scholar
Polonelli, L. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Parma
Resumo Objectives: To evaluate whether an engineered synthetic decapeptide (KP) derived from the sequence of a recombinant anti-idiotypic antibody, that represents the internal image of a Pichia anomala killer toxin, could be fungicidal in vitro and therapeutic in vivo against Paracoccidioides brasiliensis and paracoccidioidomycosis (PCM).Methods: Fungicidal activity of KP was assessed in vitro and in vivo by inhibition of colony forming units and by histological examination, 8 days after infection, of organs from mice intravenously injected with a virulent strain of P. brasiliensis (3 x 10(6) yeast cells) and intraperitoneally treated with KP (3.3 mug/g body weight, three doses), in comparison with control animals equally administered with a scrambled decapeptide (SP).Results: KP but not SP was fungicidal in vitro at 39 ng/multiply-budding yeast cell and less efficiently in its D-isomeric form (0.31 mug/multiply-budding yeast cell). It was also able to markedly reduce the fungal load in organs (liver, lung, spleen) of infected animals.Conclusions: the therapeutic effect observed opens the way for using the antifungal peptide as an alternative control of PCM in association with conventional antifungal drugs.
Assunto anti-idiotypic fragments
killer mimotopes
Idioma Inglês
Data 2004-11-01
Publicado em Journal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 54, n. 5, p. 956-958, 2004.
ISSN 0305-7453 (Sherpa/Romeo, fator de impacto)
Editor Oxford Univ Press
Extensão 956-958
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000225255000017

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