Cell cycle distribution of CD4(+) lymphocytes in HIV-1-infected subjects

Cell cycle distribution of CD4(+) lymphocytes in HIV-1-infected subjects

Autor Sauer, Mariana Melillo Autor UNIFESP Google Scholar
Kallas, Esper Georges Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Background: Apoptosis is one of the possible explanations for the progressive loss of CD4(+) T lymphocytes in infection with the human immunodeficiency virus (HIV), which may interfere with cell cycle distribution. This study evaluated the cell cycle of CD4(+) and CD8(+) lymphocytes in HIV-infected subjects and controls.Methods: Two methods to identify lymphocytes for cell cycle analysis were evaluated, magnetic beads and concurrent staining, and both were followed by propidium iodide DNA labeling. the chosen method was used to evaluate the cell cycle of lymphocytes in HIV-1-infected subjects and controls.Results: There was no significant difference between the two methods, although a higher variability was observed with the magnetic bead cell separation method. A higher proportion of cells in the S phase was observed in HIV-1 patients (2.69% vs. 1.19%, P = 0.016), coupled with a decrease in G, (96.11% vs. 98.10%, P = 0.005) in CD4(+) lymphocytes, a phenomenon not observed in CD8(+) lymphocytes. No correlation was detected between the different cell cycle phases and T-lymphocyte counts or viral load.Conclusions: the present work developed a new approach to evaluate lymphocyte cell cycle distribution, applied in the setting of HIV-1 infection. It may contribute to the understanding of the CD4(+) T-Iymphocytes depletion seen in these patients. (C) 2004 Wiley-Liss, Inc.
Assunto human immunodeficiency virus
lymphocyte
cell cycle
CD4
flow cytometry
Idioma Inglês
Data 2004-11-01
Publicado em Cytometry Part B-clinical Cytometry. Hoboken: Wiley-liss, v. 62B, n. 1, p. 46-51, 2004.
ISSN 0196-4763 (Sherpa/Romeo, fator de impacto)
Editor Wiley-Blackwell
Extensão 46-51
Fonte http://dx.doi.org/10.1002/cyto.b.20029
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000224983000005
URI http://repositorio.unifesp.br/handle/11600/27985

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