Mitochondrial alterations and apoptosis in smooth muscle from aged rats

Mitochondrial alterations and apoptosis in smooth muscle from aged rats

Author Lopes, Guiomar Silva Autor UNIFESP Google Scholar
Mora, Oswaldo Alves Autor UNIFESP Google Scholar
Cerri, Paulo Sergio Autor UNIFESP Google Scholar
Faria, Flavio Paulo de Autor UNIFESP Google Scholar
Jurkiewicz, Neide Hyppolito Autor UNIFESP Google Scholar
Jurkiewicz, Aron Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNESP
Abstract We studied changes in mitochondrial morphology and function in the smooth muscle of rat colon. Under confocal microscopy, tissues loaded with potentiometric dye displayed rapid and spontaneous depolarization. Cyclosporin A (CsA), inhibitor of the permeability transition pore (PTP), caused an increase in mitochondrial membrane potential (DeltaPsi(m)) in tissues from adult young animals. in aged rats these changes were not observed. This suggests that physiological activation of PTP in aged rats is reduced. Electron microscopy showed alterations of the mitochondrial ultrastructure in tissues from aged rats involving a decreased definition of the cristae and fragmentation of the mitochondrial membranes. We also detected an increase in apoptotic cells in the smooth muscle from aged animals. Our results show that the aging process changes PTP activity, the ability to maintain DeltaPsi(m) and mitochondrial morphology. It is suggested that these can be associated with mitochondrial damage and cell death. (C) 2004 Elsevier B.V. All rights reserved.
Keywords mitochondria
calcium
smooth muscle
aging
mitochondrial dysfunction
Language English
Date 2004-10-04
Published in Biochimica Et Biophysica Acta-bioenergetics. Amsterdam: Elsevier B.V., v. 1658, n. 3, p. 187-194, 2004.
ISSN 0005-2728 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 187-194
Origin http://dx.doi.org/10.1016/j.bbabio.2004.05.011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000224338200002
URI http://repositorio.unifesp.br/handle/11600/27978

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