Evaluation of three molecular typing techniques for nonfermentative gram-negative bacilli

Evaluation of three molecular typing techniques for nonfermentative gram-negative bacilli

Autor Silbert, S. Google Scholar
Pfaller, M. A. Google Scholar
Hollis, R. J. Google Scholar
Barth, A. L. Google Scholar
Sader, H. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Iowa
Hosp Clin Porto Alegre
Resumo OBJECTIVE: To evaluate three different DNA techniques for typing nonfermentative gram-negative bacilli isolated from Latin American hospitals.DESIGN: One hundred twenty-six nonfermentative gram-negative bacilli were typed.PARTICIPANTS: Pseudomonas aeruginosa (n = 64) and Acinetobacter baumannii (n = 42) samples were obtained from blood cultures of patients admitted to 10 medical centers in Latin America during 1998 and Stenotrophomonas maltophilia (n = 20) samples were obtained from patients admitted to the Hospital São Paulo between 1999 and 2001.METHODS: All samples were typed using automated ribotyping, PFGE, and ERIC-PCR. the discriminatory power for each technique was calculated using Hunter's generalized formula.RESULTS: All strains could be typed by automated ribotyping and ERIC-PCR, but two strains (1.6%) were not typeable by PFGE. All three techniques showed 100% reproducibility. the time to obtain the results was shorter for automated ribotyping and ERIC-PCR compared with PFGE. Likewise, the costs for ERIC-PCR and PFGE were lower than those for automated ribotyping. the interpretation of results was more complicated and more difficult with ERIC-PCR than with both PFGE and automated ribotyping. All techniques presented excellent discriminatory power for P aeruginosa (0.98). PFGE presented the highest discriminatory power (0.94) for A. baumannii, and both PFGE and ERIC-PCR showed higher discriminatory power (0.90 for both) than automated ribotyping (0.82) for S. maltophilia.CONCLUSIONS: PFGE showed the highest discriminatory power for typing these nonfermentative gram-negative bacilli. However, automated ribotyping and ERIC-PCR can provide results in a shorter time period with similar discriminatory power.
Idioma Inglês
Data 2004-10-01
Publicado em Infection Control and Hospital Epidemiology. Thorofare: Slack Inc, v. 25, n. 10, p. 847-851, 2004.
ISSN 0899-823X (Sherpa/Romeo, fator de impacto)
Editor Slack Inc
Extensão 847-851
Fonte http://dx.doi.org/10.1086/502307
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000224485700009
URI http://repositorio.unifesp.br/handle/11600/27975

Mostrar registro completo




Arquivos deste item

Arquivos Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item aparece na(s) seguinte(s) coleção(s)