Reservosome: an endocytic compartment in epimastigote forms of the protozoan Trypanosoma cruzi (Kinetoplastida : Trypanosomatidae). Correlation between endocytosis of nutrients and cell differentiation

Reservosome: an endocytic compartment in epimastigote forms of the protozoan Trypanosoma cruzi (Kinetoplastida : Trypanosomatidae). Correlation between endocytosis of nutrients and cell differentiation

Autor Figueiredo, RCBQ Google Scholar
Rosa, D. S. Google Scholar
Gomes, Y. M. Google Scholar
Nakasawa, M. Google Scholar
Soares, M. J. Google Scholar
Instituição Fiocruz MS
Universidade Federal de São Paulo (UNIFESP)
Resumo Reservosomes are large membrane-bound organelles found at the posterior end of epimastigote forms of Trypanosoma cruzi, but absent in amastigotes and trypomastigotes. We have transferred bloodstream trypomastigotes to LIT medium supplemented with gold-labelled transferrin in order to analyse, at the ultrastructural level, the occurrence of reservosomes and endocytosis during the trypomastigote to epimastigote differentiation. After 24 h, the trypomastigotes differentiated into amastigotes, which adhered to each other forming large clusters. Electron-dense vesicles were detected close to the Golgi complex in cells with intermediary characteristics between amastigotes and epimastigotes, but typical reservosomes at the posterior cell tip were still absent. Transferrin-gold complexes were observed only bound to the surface of clustered cells. After 72 h, epimastigotes were observed being released from the clusters and free-swimming epimastigotes appeared, containing electron-dense vesicles at their posterior region. Typical reservosomes, labelled with transferrin-gold, were observed only in free-swimming epimastigotes. When fully differentiated epimastigotes were incubated with transferrin-gold complexes and then processed for the immunocytochemical detection of cysteine proteinase, all reservosomes were positive for the enzyme, but co-localization of both markers did not occur in all organelles. Our data demonstrate that in T. cruzi epimastigotes endocytosis is strongly related to reservosome biogenesis during the trypomastigote to epimastigote differentiation process.
Palavra-chave endocytosis
Kinetoplastida
reservosomes
Tripanosoma cruzi
Idioma Inglês
Data de publicação 2004-10-01
Publicado em Parasitology. New York: Cambridge Univ Press, v. 129, p. 431-438, 2004.
ISSN 0031-1820 (Sherpa/Romeo, fator de impacto)
Publicador Cambridge Univ Press
Extensão 431-438
Fonte http://dx.doi.org/10.1017/S0031182004005797
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000224396000004
Endereço permanente http://repositorio.unifesp.br/handle/11600/27971

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