Antiviral activity of diterpenes isolated from the Brazilian marine alga Dictyota menstrualis against human immunodeficiency virus type 1 (HIV-1)

Antiviral activity of diterpenes isolated from the Brazilian marine alga Dictyota menstrualis against human immunodeficiency virus type 1 (HIV-1)

Author Pereira, H. S. Google Scholar
Leao-Ferreira, L. R. Google Scholar
Moussatche, N. Google Scholar
Teixeira, V. L. Google Scholar
Cavalcanti, D. N. Google Scholar
Costa, L. J. Google Scholar
Diaz, R. Google Scholar
Frugulhetti, ICPP Google Scholar
Institution Universidade Federal Fluminense (UFF)
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract The antiviral effect of the CH2Cl2/MeOH-soluble fraction from the alga Dictyota menstrualis on HIV-1 replication was evaluated in vitro. the antiretroviral activity was attributed to two diterpenes: (6R)-6-hydroxydichotoma-3,14-diene-1,17-dial, named Da-1, and (6R)-6-acetoxidichotoma-3,14-diene-1,17-dial, named AcDa-1. Da-1 or AcDa-1 were added to the culture medium of HIV-1-infected PM-1 cells at different times post-infection or during virus adsorption/penetration. the results indicated that the compounds affected an early step of the virus replicative cycle. Virus binding and entry into the host cells were evaluated in the presence of each diterpene, but no inhibitory effect was observed. To evaluate provirus DNA synthesis/integration into the host genome, the viral protease coding sequence was amplified from total cellular DNA. Proviral DNA was not detected in infected cells incubated with the diterpenes. To investigate the effect of the diterpenes on the reverse transcription of the viral genomic RNA, the recombinant HIV-1 reverse transcriptase (RT) was assayed in vitro in the presence of each diterpene. Da-1 and AcDa-1 inhibited the RNA-dependent DNA-polymerise activity of HIV-1 RT in a dose-dependent manner. Taken together, our results demonstrate that both diterpenes inhibit HIV- I RT and consequently virus replication. (C) 2004 Elsevier B.V. All rights reserved.
Keywords Dictyota menstrualis
HIV-1
diterpenes
antivirals
HIV-1 reverse transcriptase
HIV-1 RT
Language English
Date 2004-10-01
Published in Antiviral Research. Amsterdam: Elsevier B.V., v. 64, n. 1, p. 69-76, 2004.
ISSN 0166-3542 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 69-76
Origin http://dx.doi.org/10.1016/j.antiviral.2004.06.006
Access rights Closed access
Type Article
Web of Science ID WOS:000224499700008
URI http://repositorio.unifesp.br/handle/11600/27959

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