hTERT expression and prognosis in B-chronic lymphocytic leukemia

hTERT expression and prognosis in B-chronic lymphocytic leukemia

Autor Tchirkov, A. Google Scholar
Chaleteix, C. Google Scholar
Magnac, C. Google Scholar
Vasconcelos, Yuri Autor UNIFESP Google Scholar
Davi, F. Google Scholar
Michel, A. Google Scholar
Kwiatkowski, F. Google Scholar
Tournilhac, O. Google Scholar
Dighiero, G. Google Scholar
Travade, P. Google Scholar
Instituição Ctr Jean Perrin
Inst Pasteur
Hop La Pitie Salpetriere
Universidade Federal de São Paulo (UNIFESP)
Resumo Background: in B-chronic lymphocytic leukemia (B-CLL), there is a need for molecular markers to predict the evolution of this heterogeneous disease in individual patients. the level of expression of the human telomerase reverse transcriptase (hTERT) gene has been associated with disease aggressiveness in human cancers. the purpose of the present study was to examine the prognostic significance of hTERT expression in B-CLL.Patients and methods: We used real-time reverse transcription-PCR to quantitate the amount of hTERT transcripts in mononuclear blood cells from 90 B-CLL patients. in addition, samples were analyzed for somatic mutations in the immunoglobulin V (IgV) genes.Results: the expression of hTERT gene was detected in 59% of patients. the level of expression increased with advancing B-CLL stage (P = 0.0064). Patients expressing hTERT showed significantly shorter survival than hTERT-negative patients (P=0.000034), irrespective of the disease stage. On average, the level hTERT mRNA expression was seven-fold higher in the poor-prognosis B-CLL group with unmutated IgV than in the Ig-mutated group (p< 10(-7)). the level of hTERT expression discriminated the Ig-unmutated from Ig-mutated B-CLL in 89% of cases.Conclusion: Our data indicate that hTERT expression in B-CLL may serve as a molecular prognostic marker.
Assunto B-CLL
real-time reverse transcription-PCR
Idioma Inglês
Data 2004-10-01
Publicado em Annals of Oncology. Oxford: Oxford Univ Press, v. 15, n. 10, p. 1476-1480, 2004.
ISSN 0923-7534 (Sherpa/Romeo, fator de impacto)
Editor Oxford Univ Press
Extensão 1476-1480
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000224405200006

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