Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

Evaluation of fluconazole resistance mechanisms in Candida albicans clinical isolates from HIV-infected patients in Brazil

Autor Goldman, G. H. Google Scholar
Ferreira, MED Google Scholar
Marques, E. D. Google Scholar
Savoldi, M. Google Scholar
Perlin, D. Google Scholar
Park, S. Google Scholar
Martinez, PCG Google Scholar
Goldman, MHS Google Scholar
Colombo, A. L. Google Scholar
Instituição Universidade de São Paulo (USP)
Publ Hlth Res Inst
Universidade Federal de São Paulo (UNIFESP)
Resumo In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V4371, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C albicans isolates from South America. (C) 2004 Elsevier Inc. All rights reserved.
Idioma Inglês
Data de publicação 2004-09-01
Publicado em Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 50, n. 1, p. 25-32, 2004.
ISSN 0732-8893 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 25-32
Fonte http://dx.doi.org/10.1016/j.diagmicrobio.2004.04.009
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000224226200004
Endereço permanente http://repositorio.unifesp.br/handle/11600/27912

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