Pap test in a high-risk population comparison of conventional and liquid-base cytology

Pap test in a high-risk population comparison of conventional and liquid-base cytology

Author Utagawa, M. L. Google Scholar
Pereira, SMM Google Scholar
Makabe, S. Google Scholar
Maeda, MYS Google Scholar
Marques, J. A. Google Scholar
Santoro, CLF Google Scholar
Di Loreto, C. Google Scholar
Aguiar, L. S. Google Scholar
Pitolli, J. E. Google Scholar
Das Dores, G. B. Google Scholar
Castelo, A. Google Scholar
Longatto, A. Google Scholar
Institution Adolfo Lutz Inst
Perola Byignton Hosp
Digene Brasil
Universidade Federal de São Paulo (UNIFESP)
Abstract Liquid-based cytology (LBC) is believed to have better sensitivity than conventional smears (CSs) and offers the possibility to perform molecular assay. the goal of this work was to study the performance of CS and LBC in a high-risk population and to compare the results with the hybrid Capture (HC) II for high-risk human papillomavirus (HPV). Samples were collected from selected women with clinical suspicions of low genital tract lesion at Perola Biygnton Hospital (São Paulo, Brazil). After CS preparation, the brash was introduced in the endocervix and a new sample was collected and rinsed in the preservative medium of the system. the residual material was used to HC2. From 925 cases, LBC was unsatisfactory in 4 (1.51%) cases and CS was unsatisfactory in 100 cases (10.81%); among theses cases HC2(+) reactions was observed in 54 (54%) CSs and 3 (21.4%) LBCs. Considering cases with atypia of undetermined significance (squamous and glandular), 85 (77.27%) cases from LBC and 44 (86.4%) from CS were positive for HC2 assay for high-risk DNA-HPV. the difference among the methods was not significant (P < 0.38). the diagnosis improvement of LBC in comparison with CS was 86% in satisfactory samples, 92.76% in undetermined atypical lesions (including glandular), 83% in positive low-grade squamous intraepithelial lesions (LSIL+), and 86.84% in high-grade SIL+ (HSIL+). HC2+ reactions were observed in 144 CS cases and 266 LBC cases with abnormalities. Our results have showed that LBC was superior to CS in a high-risk population to detect lesions with high concordance with HC2(+) reactions; CSs also exhibit a high concordance with HC2 assay but with inferior performance to detect lesions. (C) 2004 Wiley-Liss, Inc.
Keywords liquid-base cytology
hybrid capture
HPV
DNACITOLIQ
Pap test
Language English
Date 2004-09-01
Published in Diagnostic Cytopathology. Hoboken: Wiley-liss, v. 31, n. 3, p. 169-172, 2004.
ISSN 8755-1039 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 169-172
Origin http://dx.doi.org/10.1002/dc.20118
Access rights Closed access
Type Article
Web of Science ID WOS:000223605600008
URI http://repositorio.unifesp.br/handle/11600/27903

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