False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos

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dc.contributor.author Deps, P. D.
dc.contributor.author Michalany, N. S.
dc.contributor.author Tomimori-Yamashita, J.
dc.date.accessioned 2016-01-24T12:37:20Z
dc.date.available 2016-01-24T12:37:20Z
dc.date.issued 2004-09-01
dc.identifier http://www.leprosy-ila.org/leprosyjournal/gn1/detalhe_artigo.php?id=ODE%3D&secao=CORRESPONDENCE
dc.identifier.citation International Journal of Leprosy and Other Mycobacterial Diseases. Greenville: Int Journal Leprosy, v. 72, n. 3, p. 327-330, 2004.
dc.identifier.issn 0148-916X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27902
dc.description.abstract The authors studied 66 wild nine-banded armadillos from Brazil. the ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. the AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. the authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos. en
dc.format.extent 327-330
dc.language.iso eng
dc.publisher Int Journal Leprosy
dc.relation.ispartof International Journal of Leprosy and Other Mycobacterial Diseases
dc.rights Acesso aberto
dc.title False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos en
dc.type Carta
dc.contributor.institution Fed Univ Espirito Santo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Fed Univ Espirito Santo, Dept Social Med, Infect Dis Unit, Vitoria, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Pathol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Dermatol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Pathol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Dermatol, São Paulo, Brazil
dc.identifier.doi 10.1489/0020-7349(2004)72<327:FPROTI>2.0.CO;2
dc.description.source Web of Science
dc.identifier.wos WOS:000224716500010



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