False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos

False positive reaction of the immunohistochemistry technique using anti-BCG polyclonal antibodies to identify Mycobacterium leprae in wild nine-banded armadillos

Autor Deps, P. D. Google Scholar
Michalany, N. S. Google Scholar
Tomimori-Yamashita, J. Google Scholar
Instituição Fed Univ Espirito Santo
Universidade Federal de São Paulo (UNIFESP)
Resumo The authors studied 66 wild nine-banded armadillos from Brazil. the ear samples were collected and Ziehl-Neelsen or Fite-Faraco stains were performed, as well as immunostaining using polyclonal BCG antibody, to avaluate the presence of the Mycobacterium leprae. the AFB were not detected by the Ziehl-Neelsen or Fite-Faraco staining, neither immunoexpression of the BCG marker. However, many normal structures from the ears of the nine-banded armadillos, such as condrocytes, condroblasts, fibroblasts and endothelial cells, and Gram positive bacteria cocci, showed false positive reaction by the BCG marker. the authors discuss the use of the immunohistochemical studies with the polyclonal BCG antibody to identify M. leprae antigens in wild armadillos.
Idioma Inglês
Data de publicação 2004-09-01
Publicado em International Journal of Leprosy and Other Mycobacterial Diseases. Greenville: Int Journal Leprosy, v. 72, n. 3, p. 327-330, 2004.
ISSN 0148-916X (Sherpa/Romeo, fator de impacto)
Publicador Int Journal Leprosy
Extensão 327-330
Fonte http://www.leprosy-ila.org/leprosyjournal/gn1/detalhe_artigo.php?id=ODE%3D&secao=CORRESPONDENCE
Direito de acesso Acesso aberto Open Access
Tipo Carta
Web of Science WOS:000224716500010
Endereço permanente http://repositorio.unifesp.br/handle/11600/27902

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta