Effects of carbachol on rat Sertoli cell proliferation and muscarinic acetylcholine receptors regulation: an in vitro study

Effects of carbachol on rat Sertoli cell proliferation and muscarinic acetylcholine receptors regulation: an in vitro study

Author Lucas, TFG Google Scholar
Avellar, MCW Google Scholar
Porto, C. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of the present work was to study the effect of muscarinic agonist on cell proliferation and muscarinic acetylcholine receptors (mAChRs) regulation in rat Sertoli cells. Primary cultures of Sertoli cells were obtained from 8-day and, 15-day old male Wistar rats. in proliferation assays, [methyl-H-3]thymidine incorporation in Sertoli cells from 8-day and 15-day old rats reached a plateau after 60 min of carbachol incubation and decreased after 120 min of agonist incubation. Binding studies with [N-Methyl-H-3]scopolamine ([H-3]NMS) indicated a rapid loss of cell surface mAChRs when Sertoli cells from 15-day old rats were incubated with carbachol at 35 degreesC for 2 min. This effect was temperature-dependent. When the incubation of the cells was prolonged at 35 degreesC or at 4 degreesC, after the agonist had been washed away, 94% of mAChRs were present in the cell surface after 120 min incubation at 35 degreesC. At 4 degreesC, however, a low percentage of mAChRs was detected in the cell surface. in the presence of cycloheximide, the recycling of mAChRs to the cell surface was not changed, suggesting that the appearance of mAChRs on cell surface was not dependent on de novo receptor synthesis. in conclusion, our studies indicate that the activation of mAChRs may play a role in rat Sertoli cell proliferation. These receptors may be under regulation (internalization and recycling) when cells are exposed to muscarinic cholinergic agonist. (C) 2004 Elsevier Inc. All rights reserved.
Keywords muscarinic receptors
carbachol
cell proliferation (Sertoli cells)
internalization and recycling of receptors
Language English
Date 2004-08-20
Published in Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 75, n. 14, p. 1761-1773, 2004.
ISSN 0024-3205 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1761-1773
Origin http://dx.doi.org/10.1016/j.lfs.2004.05.006
Access rights Closed access
Type Article
Web of Science ID WOS:000223012100007
URI http://repositorio.unifesp.br/handle/11600/27888

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account