Heparan sulfate and control of endothelial cell proliferation: increased synthesis during the S phase of the cell cycle and inhibition of thymidine incorporation induced by ortho-nitrophenyl-beta-D-xylose

Heparan sulfate and control of endothelial cell proliferation: increased synthesis during the S phase of the cell cycle and inhibition of thymidine incorporation induced by ortho-nitrophenyl-beta-D-xylose

Autor Moreira, C. R. Google Scholar
Lopes, C. C. Google Scholar
Cuccovia, I. M. Google Scholar
Porcionatto, M. A. Google Scholar
Dietrich, C. P. Google Scholar
Nader, H. B. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo The effect of xylosides on the synthesis of [S-35]-sulfated glycosaminoglycans by endothelial cells in culture was investigated. Orthonitrophenyl-beta-D-xylose (10(-3)M) produces a dramatic enhancement on the synthesis of heparan sulfate and chondroitin sulfate secreted to the medium (20- and 100-fold, respectively). Para-nitrophenylxyloside, at the same concentration, produces an enhancement of only 37- and 3-fold of chondroitin sulfate and heparan sulfate, respectively. These differences of action seem to be related with the higher lipophilic character of ortho-nitrophenyl-xyloside. A lower enhancement of the synthesis of the two glycosaminoglycans is also observed with 2-naphtol beta-D-xylose and cis/trans-decahydro-2-naphtol beta-D-xylose. Besides stimulating the synthesis, O-nitrophenyl-beta-D-xylose as PMA [J. Cell. Biochem. 70 (1998) 563] also inhibits [H-3]-thymidine incorporation by quiescent endothelial cells stimulated for growth by fetal calf serum (FCS). the combination of xylosides with PMA produced some cumulative effect. PMA, stimulates the synthesis of heparan sulfate mainly at G(1) phase whereas the highest enhancement of synthesis produced by the xylosides is in the S phase of the endothelial cell cycle. (C) 2004 Elsevier B.V. All rights reserved.
Palavra-chave heparan sulfate and growth factors
heparan sulfate and nitrophenylxylosides
cell cycle and xylosides
inhibition of DNA synthesis and o-nitrophenylxyloside
xylosides and heparan sulfate synthesis
Idioma Inglês
Data de publicação 2004-08-04
Publicado em Biochimica Et Biophysica Acta-general Subjects. Amsterdam: Elsevier B.V., v. 1673, n. 3, p. 178-185, 2004.
ISSN 0304-4165 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 178-185
Fonte http://dx.doi.org/10.1016/j.bbagen.2004.04.014
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000223122000009
Endereço permanente http://repositorio.unifesp.br/handle/11600/27880

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