Leucine-rich amelogenin peptide: A candidate signaling molecule during cementogenesis

Leucine-rich amelogenin peptide: A candidate signaling molecule during cementogenesis

Autor Boabaid, F. Google Scholar
Gibson, C. W. Google Scholar
Kuehl, M. A. Google Scholar
Berry, J. E. Google Scholar
Snead, M. L. Google Scholar
Nociti, F. H. Google Scholar
Katchburian, E. Autor UNIFESP Google Scholar
Somerman, M. J. Google Scholar
Instituição Univ Washington
Univ Michigan
Universidade Federal de São Paulo (UNIFESP)
Univ Penn
Univ So Calif
Universidade Estadual de Campinas (UNICAMP)
Resumo Background: Cementum is a critical mineralized tissue; however, control of its formation remains undefined. One hypothesis is that enamel matrix proteins/peptides secreted by ameloblasts and/or epithelial rest cells contribute to the control of cementum formation via epithelial-mesenchymal interactions. Here, we focused on determining whether or not leucine-rich amelogenin peptide (LRAP), translated from an alternatively spliced amelogenin RNA, altered cementoblast behavior.Methods: Immortalized murine cementoblasts (OCCM-30) were exposed to LRAP and evaluated for: 1) proliferative activity; 2) gene expression using Northern blot for Cbfa1 (core binding factor alpha-1); OCN (osteocalcin), OPN (osteopontin), and real-time reverse transcription-polymerase chain reaction (RT-PCR) for OPG (osteoprotegerin); and RANKL (receptor activator of NF-kappaB ligand); 3) signaling pathway using inhibitors of PKA (THFA), PKC (GF109203X), and MAPK (UO126); and 4) mineralization evaluated by von Kossa and Alizarin-red.Results: LRAP had no effect on cell proliferation up to 6 days, with a decrease in cell growth observed at the highest dose by 9 days versus untreated cells. LRAP down regulated OCN and up regulated OPN in a dose- and time-response fashion, and inhibited the capacity of mineral nodule formation. Transcripts for OPG were increased in LRAP-treated cells compared to control, but RANKL mRNA levels were not affected. Core binding factor alpha (Cbfa) mRNA, expressed constitutively, was not affected by LRAP. Signaling pathway assays suggested involvement of the MAPK pathway, since the addition of the MAPK inhibitor suppressed OPN expression in LRAP-treated cells.Conclusion: Leucine-rich amelogenin peptide appears to have a direct effect on cementoblast activity that may prove significant during development as well as in regeneration of periodontal tissues.
Palavra-chave cementoblasts
dental cementum
peptides
leucine-rich amelogenin
periodontal regeneration
Idioma Inglês
Data de publicação 2004-08-01
Publicado em Journal of Periodontology. Chicago: Amer Acad Periodontology, v. 75, n. 8, p. 1126-1136, 2004.
ISSN 0022-3492 (Sherpa/Romeo, fator de impacto)
Publicador Amer Acad Periodontology
Extensão 1126-1136
Fonte http://dx.doi.org/10.1902/jop.2004.75.8.1126
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000223509100014
Endereço permanente http://repositorio.unifesp.br/handle/11600/27877

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