Catecholamine effects on human melanoma cells evoked by alpha(1)-adrenoceptors

Catecholamine effects on human melanoma cells evoked by alpha(1)-adrenoceptors

Author Scarparo, A. C. Google Scholar
Sumida, D. H. Google Scholar
Patrao, MTCC Google Scholar
Avellar, MCW Google Scholar
Visconti, M. A. Google Scholar
Castrucci, AMD Google Scholar
Institution Universidade de São Paulo (USP)
Univ Nacl Estadual São Paulo
Universidade Federal de São Paulo (UNIFESP)
Abstract The biological effects of catecholamines in mammalian pigment cells are poorly understood. Our previous results showed the presence of alpha(1)-adrenoceptors in SK-Mel 23 human melanoma cells. the aims of this work were to (1) characterize catecholamine effects on proliferation, tyrosinase activity and expression, (2) identify the alpha(1)-adrenoceptor subtypes, and (3) verify whether chronic norepinephrine (NE) treatment modified the types and/or pharmacological characteristics of adrenoceptors present in SK-Mel 23 human melanoma cells. Cells treated with the alpha(1)-adrenergic agonist, phenylephrine (PHE, 10(-5) or 10(-4) M), for 24-72 h, exhibited decreased cell proliferation and enhanced tyrosinase activity, but unaltered tyrosinase expression as compared with the control. the proliferation and tyrosinase activity responses were inhibited by the alpha(1)-adrenergic antagonist prazosin, suggesting they were evoked by alpha(1)-adrenoceptors. the presence of actinomycin D, a transcription inhibitor, did not diminish PHE-induced effects. RT-PCR assays, followed by cloning and sequencing, demonstrated the presence of alpha(1A)- and alpha(1B)-adrenoceptor subtypes. NE-treated cells (24 or 72 h) were used in competition assays, and showed no significant change in the competition curves of alpha(1)-adrenoceptors as compared with control curves. Other adrenoceptor subtypes were not identified in these cells, and NE pretreatment did not induce their expression. in conclusion, the activation of SK-Mel 23 human melanoma alpha(1)-adrenoceptors elicit biological effects, such as proliferation decrease and tyrosinase activity increase. Desensitization or expression of other adrenoceptor subtypes after chronic NE treatment were not observed.
Keywords catecholamines
SK-Mel 23 melanoma cells
cell proliferation
tyrosinase activity
Language English
Date 2004-08-01
Published in Archives of Dermatological Research. New York: Springer, v. 296, n. 3, p. 112-119, 2004.
ISSN 0340-3696 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 112-119
Access rights Closed access
Type Article
Web of Science ID WOS:000223374900003

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