A transcript finishing initiative for closing gaps in the human transcriptome

A transcript finishing initiative for closing gaps in the human transcriptome

Autor Sogayar, M. C. Google Scholar
Camargo, A. A. Google Scholar
Bettoni, F. Google Scholar
Carraro, D. M. Google Scholar
Pires, L. C. Google Scholar
Parmigiani, M. B. Google Scholar
Ferreira, E. N. Google Scholar
Moreira, E. S. Google Scholar
Latorre, MRDO Google Scholar
Simpson, AJG Google Scholar
Cruz, L. O. Google Scholar
Degaki, T. L. Google Scholar
Festa, F. Google Scholar
Massirer, K. B. Google Scholar
Camargo, F. Google Scholar
Camargo, L. P. Google Scholar
Cunha, MAV Google Scholar
De Souza, S. J. Google Scholar
Faria, M. Google Scholar
Giuliatti, S. Google Scholar
Kopp Google Scholar
Oliviera, PSL Google Scholar
Paiva, Paulo Bandiera Autor UNIFESP Google Scholar
Pereira, A. A. Google Scholar
Pinheiro, D. G. Google Scholar
Puga, R. D. Google Scholar
Souza, JES Google Scholar
Albuquerque, D. M. Google Scholar
Andrade, Luís EC Autor UNIFESP Google Scholar
Baia, G. S. Google Scholar
Briones, Marcelo Ribeiro da Silva Autor UNIFESP Google Scholar
Cavaleiro-Luna, AMS Google Scholar
Cerutti, Janete Maria Autor UNIFESP Google Scholar
Costa, F. F. Google Scholar
Costanzi-Strauss, Eugenia Autor UNIFESP Google Scholar
Espreafico, E. M. Google Scholar
Ferrasi, A. C. Google Scholar
Ferro, E. S. Google Scholar
Fortes, MAHZ Google Scholar
Furchi, JRF Google Scholar
Gianella-Neto, D. Google Scholar
Goldman, G. H. Google Scholar
Goldman, MHS Google Scholar
Gruber, A. Google Scholar
Guimaraes, G. S. Google Scholar
Hackel, C. Google Scholar
Henrique-Silva, F. Google Scholar
Kimura, E. T. Google Scholar
Leoni, S. G. Google Scholar
Macedo, C. Google Scholar
Malnic, B. Google Scholar
Manzini, C. V. Google Scholar
Marie, SKN Google Scholar
Martinez-Rossi, N. M. Google Scholar
Menossi, M. Google Scholar
Miracca, E. C. Google Scholar
Nagai, M. A. Google Scholar
Nobrega, F. G. Google Scholar
Nobrega, M. P. Google Scholar
Oba-Shinjo, S. M. Google Scholar
Oliviera, M. K. Google Scholar
Orabona, G. M. Google Scholar
Otsuke, A. Y. Google Scholar
Paco-Larson, M. L. Google Scholar
Paixao, BMC Google Scholar
Pandolfi, JRC Google Scholar
Pardini, MIMC Google Scholar
Passos-Bueno, M. R. Google Scholar
Passos, Geraldo AS Autor UNIFESP Google Scholar
Pesquero, João Bosco Autor UNIFESP Google Scholar
Pessoa, Juliana G. Autor UNIFESP Google Scholar
Rahal, P. Google Scholar
Rainho, C. A. Google Scholar
Reis, C. P. Google Scholar
Ricca, Tatiana I. Autor UNIFESP Google Scholar
Rodriguez, V Google Scholar
Rogatto, SR Google Scholar
Romano, C. M. Google Scholar
Romeiro, J. G. Google Scholar
Rossi, A. Google Scholar
Sa, R. G. Google Scholar
Sales, M. M. Google Scholar
SantAnna, S. C. Google Scholar
Santarosa, P. L. Google Scholar
Segato, F. Google Scholar
Silva, W. A. Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Silva, Neusa Pereira da Autor UNIFESP Google Scholar
Soares-Costa, A. Google Scholar
Sonati, M. F. Google Scholar
Strauss, B. E. Google Scholar
Tajara, E. H. Google Scholar
Valentini, SR Google Scholar
Villanova, Fabiula Elizabeth Autor UNIFESP Google Scholar
Ward, L. S. Google Scholar
Zanette, D. L. Google Scholar
Ludwig-FAPESP Transcript Finishing In Google Scholar
Instituição Universidade de São Paulo (USP)
Ludwig Inst Canc Res
Univ Ribeirao Preto
Inst Ludwig
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Carlos (UFSCar)
Univ Vale Paraiba
São Paulo State Univ
Univ Estadual Paulista
Resumo We report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST Clusters, mapped against the genomic sequence. Each pair of EST Clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. the structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. the TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted Set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms.
Idioma Inglês
Data de publicação 2004-07-01
Publicado em Genome Research. Cold Spring Harbor: Cold Spring Harbor Lab Press, Publications Dept, v. 14, n. 7, p. 1413-1423, 2004.
ISSN 1088-9051 (Sherpa/Romeo, fator de impacto)
Publicador Cold Spring Harbor Lab Press, Publications Dept
Extensão 1413-1423
Fonte http://dx.doi.org/10.1101/gr.2111304
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000222434200023
Endereço permanente http://repositorio.unifesp.br/handle/11600/27803

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