Hyperresponsiveness of neutrophils from gp 91(phox) deficient patients to lipopolysaccharide and serum amyloid A

Hyperresponsiveness of neutrophils from gp 91(phox) deficient patients to lipopolysaccharide and serum amyloid A

Autor Hatanaka, E. Google Scholar
Carvalho, BTC Google Scholar
Condino-Neto, A. Google Scholar
Campa, A. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Resumo We demonstrate here that neutrophils from chronic granulomatous disease (CGD) patients release larger amounts of interleukin-8 (IL-8) and tumor necrosis factor alpha (TNF-alpha) than neutrophils from control subjects. Incremental cytokine production was observed under both basal and stimulated conditions in neutrophils from two CGD (gp 91(phox)) patients. the basal production of IL-8 was over seven-fold greater in CGD patients. the two samples assayed showed 3- and 10-fold increases in TNF-alpha. Basically, the same magnitude of increment was observed in lypopolysaccharide (LPS) and serum amyloid A protein (SAA)-stimulated cells. We also found that the levels of SAA and IL-8 were higher in the serum of CGD patients than the levels found in the serum of healthy donors. the increased responsiveness of neutrophils from CGD patients may be closely related with a deficiency in the assembly of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase enzyme system, or it may be due to a frequent inflammatory condition in these patients. in the latter case, the increased serum levels of systemic inflammatory factors, among them SAA, would contribute to the sustained accumulation and activation of phagocytes. Whatever the origin, the excessive production of cytokines may lead to inappropriate activation and tissue injury and even to increased susceptibility to invasive microorganisms, impairing the quality life of CGD patients. (C) 2004 Elsevier B.V. All rights reserved.
Palavra-chave IL-8
TNF-alpha
CGD
neutrophils
SAA
Idioma Inglês
Data de publicação 2004-06-15
Publicado em Immunology Letters. Amsterdam: Elsevier B.V., v. 94, n. 1-2, p. 43-46, 2004.
ISSN 0165-2478 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 43-46
Fonte http://dx.doi.org/10.1016/j.imlet.2004.04.016
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000222819300006
Endereço permanente http://repositorio.unifesp.br/handle/11600/27795

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