Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein

Epitope mapping of a single repetitive unit of the B13 Trypanosoma cruzi antigen as fusions to Escherichia coli LamB protein

Autor Pereira, Catia Mendes Autor UNIFESP Google Scholar
Franco da Silveira, José Autor UNIFESP Google Scholar
Duranti, M. Google Scholar
Cunha-Neto, E. Google Scholar
Zingales, B. Google Scholar
Castilho, Beatriz Amaral de Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo B13, one of the immunodominant antigens of Trypanosoma cruzi, is composed of repeats of a 12-amino-acid motif. Using synthetic peptides, the sequence FGQAAAGDK was previously shown to contain the B13 immunodominant epitope recognized by chagasic patients sera. To investigate the effects of neighboring sequences in the immunodominance, we tested serum recognition of two B13 sequences fused to LamB. GDKPSPFGQAAA-LamB and FGQAAAGDKPSP-LamB were recognized, respectively, by 15% and 80% of 80 sera reactive to B13 antigen. Recognition of FGQAAAGDKPSP-LamB was inhibited by AAAGDK-containing synthetic peptides. FGQAAAGDKPSP-LamB competed with a B13 recombinant protein containing 16.6 repeats for binding to chagasic antibodies. These results strengthen previous conclusions on the immunodominant epitope of B13 and provide a comparison of two methods for epitope mapping. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies.
Palavra-chave Trypanosoma cruzi
immunodominant repetitive antigen
B13 antigen
B cell epitope mapping
Escherichia coli LamB protein
Idioma Inglês
Data de publicação 2004-06-15
Publicado em Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 235, n. 2, p. 237-242, 2004.
ISSN 0378-1097 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 237-242
Fonte http://dx.doi.org/10.1016/j.femsle.2004.04.040
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000222144400004
Endereço permanente http://repositorio.unifesp.br/handle/11600/27792

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