Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability

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dc.contributor.author Maccariello, E.
dc.contributor.author Dalboni, Maria Aparecida [UNIFESP]
dc.contributor.author Draibe, S. A.
dc.contributor.author Rocha, E.
dc.contributor.author Santos, OFP dos
dc.contributor.author Santos, B.
dc.contributor.author Cendoroglo, M.
dc.date.accessioned 2016-01-24T12:37:12Z
dc.date.available 2016-01-24T12:37:12Z
dc.date.issued 2004-06-01
dc.identifier http://dx.doi.org/10.1111/j.1525-1594.2004.07284.x
dc.identifier.citation Artificial Organs. Malden: Blackwell Publishing Inc, v. 28, n. 6, p. 571-576, 2004.
dc.identifier.issn 0160-564X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27789
dc.description.abstract It has previously been shown that the mixture of bicarbonate and calcium in the solutions used for continuous renal replacement therapy led to crystalization and significant changes in calcium concentration and pH. the aim of this study was to investigate the impact of bicarbonate/calcium and lactate/calcium solutions for Continuous Renal Replacement Therapies (CRRT) on the viability and function of polymorphonuclear cells (PMN). We tested four customized bicarbonate buffered solutions: single bag (bicarbonate and calcium mixed 24 h before testing), double bag (mixed immediately before testing), filtered single bag and double bag solutions, and a commercial lactate buffered solution. Blood from 6 volunteers was incubated with the solutions for 30 min followed by PMN isolation. After overnight incubation, viability, phagocytosis, and peroxide production by PMN were determined by flow cytometry. There was no difference between the test solutions with respect to PMN viability and function. Therefore, the presence of microcrystals and the consequent changes in electrolyte concentrations do not seem to impair PMN function. en
dc.format.extent 571-576
dc.language.iso eng
dc.publisher Blackwell Publishing Inc
dc.relation.ispartof Artificial Organs
dc.rights Acesso restrito
dc.subject dialysis en
dc.subject hemofiltration en
dc.subject solutions en
dc.subject polymorphonuclear cells en
dc.subject viability en
dc.subject function en
dc.title Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal Fluminense (UFF)
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institution Albert Einstein Jewish Hosp
dc.description.affiliation Universidade Federal de São Paulo, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.description.affiliation Univ Fed Fluminense, Div Nephrol, Rio de Janeiro, Brazil
dc.description.affiliation Fed Univ Rio de Janeiro, HUCFF, Multidisciplinary Lab, Rio de Janeiro, Brazil
dc.description.affiliation Albert Einstein Jewish Hosp, Dialysis Unit, São Paulo, Brazil
dc.description.affiliation Albert Einstein Jewish Hosp, Intens Care Unit, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFESP, Div Nephrol, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1525-1594.2004.07284.x
dc.description.source Web of Science
dc.identifier.wos WOS:000221532200009



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