Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability

Effects of customized bicarbonate buffered solutions for continuous renal replacement therapies on polymorphonuclear leukocytes function and viability

Author Maccariello, E. Google Scholar
Dalboni, Maria Aparecida Autor UNIFESP Google Scholar
Draibe, S. A. Google Scholar
Rocha, E. Google Scholar
Santos, OFP dos Google Scholar
Santos, B. Google Scholar
Cendoroglo, M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal Fluminense (UFF)
Universidade Federal do Rio de Janeiro (UFRJ)
Albert Einstein Jewish Hosp
Abstract It has previously been shown that the mixture of bicarbonate and calcium in the solutions used for continuous renal replacement therapy led to crystalization and significant changes in calcium concentration and pH. the aim of this study was to investigate the impact of bicarbonate/calcium and lactate/calcium solutions for Continuous Renal Replacement Therapies (CRRT) on the viability and function of polymorphonuclear cells (PMN). We tested four customized bicarbonate buffered solutions: single bag (bicarbonate and calcium mixed 24 h before testing), double bag (mixed immediately before testing), filtered single bag and double bag solutions, and a commercial lactate buffered solution. Blood from 6 volunteers was incubated with the solutions for 30 min followed by PMN isolation. After overnight incubation, viability, phagocytosis, and peroxide production by PMN were determined by flow cytometry. There was no difference between the test solutions with respect to PMN viability and function. Therefore, the presence of microcrystals and the consequent changes in electrolyte concentrations do not seem to impair PMN function.
Keywords dialysis
hemofiltration
solutions
polymorphonuclear cells
viability
function
Language English
Date 2004-06-01
Published in Artificial Organs. Malden: Blackwell Publishing Inc, v. 28, n. 6, p. 571-576, 2004.
ISSN 0160-564X (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Inc
Extent 571-576
Origin http://dx.doi.org/10.1111/j.1525-1594.2004.07284.x
Access rights Closed access
Type Article
Web of Science ID WOS:000221532200009
URI http://repositorio.unifesp.br/handle/11600/27789

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