Binding of laminin to Paracoccidioides brasiliensis induces a less severe pulmonary paracoccidioidomycosis caused by virulent and low-virulence isolates

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dc.contributor.author Andre, D. C.
dc.contributor.author Lopes, J. D.
dc.contributor.author Franco, M. F.
dc.contributor.author Vaz, CAC
dc.contributor.author Calich, VLG
dc.date.accessioned 2016-01-24T12:37:08Z
dc.date.available 2016-01-24T12:37:08Z
dc.date.issued 2004-05-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2004.02.010
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 6, n. 6, p. 549-558, 2004.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27733
dc.description.abstract The pathogenic fungus Parracoccidioides brasiliensis is the causative agent of paraeoccidioidomycosis (PCM). This pulmonary mycosis, acquired by inhalation of airborne propagules, may disseminate to several internal organs and tissues, leading to severe disease. Adhesion to host cell components is the first step involved in dissemination of pathogens. Previous studies showed that laminin, the most abundant glycoprotein of the basement membrane, binds to P. brasiliensis yeast cells, enhancing their pathogenicity in the hamster testicle model. As PCM is primarily a pulmonary infection, we studied the influence of previous treatment of yeast cells with laminin on the course of the intratracheal infection of resistant and susceptible mice using high-virulence (Pb18) and low-virulence (Pb265) P. brasiliensis isolates. Laminin treatment did not alter fungal loads, delayed-type hypersensitivity reactions, levels of pulmonary cytokines and production of specific antibodies in any group of Pb18-infected mice. However, early in the infection, a less intense inflammatory reaction was detected in the lungs of the laminin-treated groups. in addition, laminin treatment of P6265 resulted in a less severe infection as revealed by the lower fungal loads recovered from lungs. Antibody and cytokine levels, however, did not change after laminin treatment. Altogether, our results demonstrate that laminin binding to yeast cells diminishes P. brasiliensis pathogenicity. the lower inflammatory response observed with the virulent isolate and the decreased pulmonary fungal burden with the low-virulence isolate indicate an inhibitory effect of laminin treatment on P. brasiliensis infectivity and interaction with pulmonary host cells or extracellular matrix proteins. (C) 2004 Elsevier SAS. All rights reserved. en
dc.format.extent 549-558
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso restrito
dc.subject Paracoccidioides brasiliensis en
dc.subject laminin en
dc.subject pathogenicity en
dc.subject immunity en
dc.title Binding of laminin to Paracoccidioides brasiliensis induces a less severe pulmonary paracoccidioidomycosis caused by virulent and low-virulence isolates en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Inst Ciencias Biomed, Dept Immunol, BR-05508900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Fac Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Fac Med, Dept Patol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Fac Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Fac Med, Dept Patol, São Paulo, Brazil
dc.identifier.doi 10.1016/j.micinf.2004.02.010
dc.description.source Web of Science
dc.identifier.wos WOS:000221835000005



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