The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy

The synthesis and distribution of the kinin B1 and B2 receptors are modified in the hippocampus of rats submitted to pilocarpine model of epilepsy

Author Arganaraz, G. A. Google Scholar
Silva, J. A. Google Scholar
Perosa, SR Google Scholar
Pessoa, L. G. Google Scholar
Carvalho, F. F. Google Scholar
Bascands, J. L. Google Scholar
Bader, M. Google Scholar
Trindade, E. D. Google Scholar
Amado, D. Google Scholar
Cavalheiro, E. A. Google Scholar
Pesquero, J. B. Google Scholar
Naffah-Mazzacoratti, M. D. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Fac Med Toulouse
Max Delbruck Ctr
Abstract Kinins, a special class of polypeptides, are represented by bradykinin (BK), kallidin (Lys-BK), as well as their metabolites. the biological actions of these polypeptides binding on their receptors (B1 and B2) have been related to inflammation process, cytokines action, glutamate release and prostaglandins production. Usually, kinin B1 receptor is not expressed at a significant level under physiologic conditions in most tissues, but its expression is induced by injury, or upon exposure in vivo or in vitro to pro-inflammatory mediators. the kinin B2 receptor subtype is constitutively and widely expressed throughout the central and peripheral nervous system. These data raise the possibility for de novo expression of those receptors during the temporal lobe epilepsy (TLE), which has been related to cell death, gliosis and hippocampal reorganization. To correlate kinin system and TLE, adult male Wistar rats were submitted to pilocarpine model of epilepsy. the hippocampi were removed 6 h, 5 and 60 days after status epilepticus (SE) onset. the collected tissues were used to study the expression of kinin B1 and B2 mRNA receptors, using Real-Time PCR. Immunohistochemistry assay was also employed to visualize kinin B1 and B2 distribution in the hippocampus. the results show increased kinin B1 and B2 mRNA levels during acute, silent and chronic periods and changes in the kinin B1 and B2 receptors distribution. in addition, the immunoreactivity against kinin B1 receptor was increased mainly during the silent period, where neuron clusters of could be visualized. the kinin B2 receptor immunoreactivity also showed augmentation but mainly during the acute and silent periods. Our results suggest that kinin B1 and B2 receptors play an important role in the epileptic phenomena. (C) 2004 Elsevier B.V. All rights reserved.
Keywords kinin B1 and B2 receptors
temporal lobe epilepsy
pilocarpine
hippocampus
plasticity
Status epilepticus
Language English
Date 2004-04-23
Published in Brain Research. Amsterdam: Elsevier B.V., v. 1006, n. 1, p. 114-125, 2004.
ISSN 0006-8993 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 114-125
Origin http://dx.doi.org/10.1016/j.brainres.2003.12.050
Access rights Closed access
Type Article
Web of Science ID WOS:000220822400013
URI http://repositorio.unifesp.br/handle/11600/27711

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