Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages

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dc.contributor.author Fernandes, A. B.
dc.contributor.author Mortara, R. A.
dc.date.accessioned 2016-01-24T12:37:05Z
dc.date.available 2016-01-24T12:37:05Z
dc.date.issued 2004-04-01
dc.identifier http://dx.doi.org/10.1016/j.micinf.2004.01.009
dc.identifier.citation Microbes and Infection. Amsterdam: Elsevier B.V., v. 6, n. 5, p. 460-467, 2004.
dc.identifier.issn 1286-4579
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27695
dc.description.abstract In order to invade mammalian cells, Trypanosoma cruzi infective forms cause distinct rearrangements of membrane and host cell cytoskeletal components. Rho GTPases have been shown to regulate three separate signal transduction pathways, linking plasma membrane receptors to the assembly of distinct actin filament structures. Here, we examined the role of Rho GTPases on the interaction between different T cruzi infective forms of strains from the two major phylogenetic lineages with nonpolarized MDCK cells transfected with different Rho GTPase constructs. We compared the infectivity of amastigotes isolated from infected cells (intracellular amastigotes) with forms generated from the axenic differentiation of trypomastigotes (extracellular amastigotes), and also with metacyclic trypomastigotes.No detectable effect of GTPase expression was observed on metacyclic trypomastigote invasion and parasites of Y and CL (T cruzi 11) strains invaded to similar degrees all MDCK transfectants, and were more infective than either G or Tulahuen (T cruzi I) strains. Intracellular amastigotes were complement sensitive and showed very low infectivity towards the different transfectants regardless of the parasite strain. Complement-resistant T cruzi I extracellular amastigotes, especially of the G strain, were more infective than T cruzi 11 parasites, particularly for the Rac1V12 constitutively active GTPase transfectant. the fact that in Rac1N17 dominant-negative cells, the invasion of G strain extracellular amastigotes was specifically inhibited suggested an important role for Rac1 in this process. (C) 2004 Elsevier SAS. All rights reserved. en
dc.format.extent 460-467
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Microbes and Infection
dc.rights Acesso restrito
dc.subject trypanosoma cruzi en
dc.subject cell invasion en
dc.subject Rho GTPases en
dc.subject amastigotes en
dc.subject trypomastigotes en
dc.subject phylogenetic lineages en
dc.title Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, UNIFEST, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFEST, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1016/j.micinf.2004.01.009
dc.description.source Web of Science
dc.identifier.wos WOS:000221494200006



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