A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression

A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression

Author Cerutti, Janete Maria Autor UNIFESP Google Scholar
Delcelo, Rosana Autor UNIFESP Google Scholar
Amadei, Marcelo João Autor UNIFESP Google Scholar
Nakabashi, Claudia Autor UNIFESP Google Scholar
Maciel, Rui Monteiro de Barros Autor UNIFESP Google Scholar
Peterson, Bercedis Google Scholar
Shoemaker, Jennifer Google Scholar
Riggins, Gregory J. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Duke Univ
Johns Hopkins Univ
Abstract Accurate diagnosis of thyroid tumors is challenging. A particular problem is distinguishing between follicular thyroid carcinoma (FTC) and benign follicular thyroid adenoma (FTA), where histology of fine-needle aspirates is not conclusive. It is often necessary to remove healthy thyroid to rule out carcinoma. in order to find markers to improve diagnosis, we quantified gene transcript expression from FTC, FTA, and normal thyroid, revealing 73 differentially expressed transcripts (Pless than or equal to.0001). Using an independent set of 23 FTCs, FTAs, and matched normal thyroids, 17 genes with large expression differences were tested by real-time RT-PCR. Four genes (DDIT3, ARG2, ITM1, and C1orf24) differed between the two classes FTC and FTA, and a linear combination of expression levels distinguished FTC from FTA with an estimated predictive accuracy of 0.83. Furthermore, immunohistochemistry for DDIT3 and ARG2 showed consistent staining for carcinoma in an independent set 59 follicular tumors (estimated concordance, 0.76; 95% confidence interval, [0.59, 0.93]). A simple test based on a combination of these markers might improve preoperative diagnosis of thyroid nodules, allowing better treatment decisions and reducing long-term health costs.
Language English
Date 2004-04-01
Published in Journal of Clinical Investigation. Ann Arbor: Amer Soc Clinical Investigation Inc, v. 113, n. 8, p. 1234-1242, 2004.
ISSN 0021-9738 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Clinical Investigation Inc
Extent 1234-1242
Origin http://dx.doi.org/10.1172/JCI200419617
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000221365100023
URI http://repositorio.unifesp.br/handle/11600/27686

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