Phosphorylation of translation initiation factor eIF2 alpha in the brain during pilocarpine-induced status epilepticus in mice

Phosphorylation of translation initiation factor eIF2 alpha in the brain during pilocarpine-induced status epilepticus in mice

Author Carnevalli, L. S. Google Scholar
Pereira, C. M. Google Scholar
Longo, B. M. Google Scholar
Jaqueta, C. B. Google Scholar
Avedissian, M. Google Scholar
Mello, LEAM Google Scholar
Castilho, B. A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In this work, we show extensive phosphorylation of the alpha subunit of translation initiation factor 2 (eIF2alpha) occurring in the brain of mice subjected to 30 min of status epilepticus induced by pilocarpine. eIF2alpha(P) immumoreactivity was detected in the hippocampal pyramidal layer CA1 and CA3, cortex layer V, thalamus and amygdala. After 2 It of recovery, there was a marked decrease in total brain eIF2alpha(P), with the cortex layer V showing the most pronounced loss of anti-eIF2alpha(P) labeling, whereas the CA1 subregion had a significant increase in eIF2alpha(P). These results indicate that inhibition of protein synthesis in experimental models of epilepsy might be due to low levels of eIF2-GTP caused by the phosphorylation of eIF2alpha, and suggest that translational control may contribute to cell fate in the affected areas. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Keywords eIF2 alpha phosphorylation
epilepsy
pilocarpine
translation initiation
Language English
Date 2004-03-11
Published in Neuroscience Letters. Clare: Elsevier Sci Ireland Ltd, v. 357, n. 3, p. 191-194, 2004.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 191-194
Origin http://dx.doi.org/10.1016/j.neulet.2003.12.093
Access rights Closed access
Type Article
Web of Science ID WOS:000220125800009
URI http://repositorio.unifesp.br/handle/11600/27673

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