Light microscopy and computer three-dimensional reconstruction of the blood capillaries of the enamel organ of rat molar tooth germs

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dc.contributor.author Cerri, Paulo Sergio
dc.contributor.author Faria, Flavio Paulo de
dc.contributor.author Villa, Ricardo Gazoni [UNIFESP]
dc.contributor.author Katchburian, Eduardo [UNIFESP]
dc.date.accessioned 2016-01-24T12:34:18Z
dc.date.available 2016-01-24T12:34:18Z
dc.date.issued 2004-03-01
dc.identifier http://dx.doi.org/10.1111/j.0021-8782.2004.00268.x
dc.identifier.citation Journal of Anatomy. Oxford: Blackwell Publishing Ltd, v. 204, n. 3, p. 191-195, 2004.
dc.identifier.issn 0021-8782
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/27637
dc.description.abstract We performed a light microscope and a computer three-dimensional reconstruction study of serial sections of the molar enamel organ of 3- and 5-day-old rats perfused with Indian ink through the arterial system. the tooth germs were fixed in Bouin's solution, embedded in paraffin, sectioned and stained with haematoxylin and eosin. for the three-dimensional reconstruction, light micrographs of the serial sections were digitized, and aligned using the serial EM Align software downloaded from http://synapses.bu.edu/tools/. After alignment, the boundaries of the India-ink-filled blood vessels were manually traced with a mouse using the software IGL trace (version 1.26b), also downloaded from the above website. After tracing, a three-dimensional representation of the blood vessel contours was generated in a VRML format and visualized with the help of the software Cortona Web3D viewer (version 4.0) downloaded from http://www.parallelgraphics.com/products/cortona. Our results showed that in regions where ameloblasts are polarized the capillaries are arranged in three distinct levels: (1) penetrating and leaving capillaries in relation to the outer enamel epithelium; (2) capillaries crossing and branching inside the stellate reticulum; and (3) capillaries branching and anastomosing profusely within the stratum intermedium, thereby forming an extensive capillary plexus intimately associated with the cells of the stratum intermedium. the existence of a conspicuous capillary plexus intermingled with cells of the stratum intermedium, as shown in our results, suggests that some molecules produced by cells of the stratum intermedium could be released into the capillary plexus and thereafter carried to the dental follicle. en
dc.format.extent 191-195
dc.language.iso eng
dc.publisher Blackwell Publishing Ltd
dc.relation.ispartof Journal of Anatomy
dc.rights Acesso aberto
dc.subject capillaries en
dc.subject computer reconstruction en
dc.subject enamel organ en
dc.subject stellate reticulum en
dc.subject tooth germ en
dc.title Light microscopy and computer three-dimensional reconstruction of the blood capillaries of the enamel organ of rat molar tooth germs en
dc.type Artigo
dc.contributor.institution UNESP
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation UNESP, Sch Dent, Dept Morphol, BR-14801903 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, UNIFESP, Sch Med, Ctr Electron Microscopy, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, UNIFESP, Sch Med, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFESP, Sch Med, Ctr Electron Microscopy, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFESP, Sch Med, Dept Morphol, São Paulo, Brazil
dc.identifier.doi 10.1111/j.0021-8782.2004.00268.x
dc.description.source Web of Science
dc.identifier.wos WOS:000189258200004



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