Light microscopy and computer three-dimensional reconstruction of the blood capillaries of the enamel organ of rat molar tooth germs

Light microscopy and computer three-dimensional reconstruction of the blood capillaries of the enamel organ of rat molar tooth germs

Author Cerri, Paulo Sergio Google Scholar
Faria, Flavio Paulo de Google Scholar
Villa, Ricardo Gazoni Autor UNIFESP Google Scholar
Katchburian, Eduardo Autor UNIFESP Google Scholar
Institution UNESP
Universidade Federal de São Paulo (UNIFESP)
Abstract We performed a light microscope and a computer three-dimensional reconstruction study of serial sections of the molar enamel organ of 3- and 5-day-old rats perfused with Indian ink through the arterial system. the tooth germs were fixed in Bouin's solution, embedded in paraffin, sectioned and stained with haematoxylin and eosin. for the three-dimensional reconstruction, light micrographs of the serial sections were digitized, and aligned using the serial EM Align software downloaded from http://synapses.bu.edu/tools/. After alignment, the boundaries of the India-ink-filled blood vessels were manually traced with a mouse using the software IGL trace (version 1.26b), also downloaded from the above website. After tracing, a three-dimensional representation of the blood vessel contours was generated in a VRML format and visualized with the help of the software Cortona Web3D viewer (version 4.0) downloaded from http://www.parallelgraphics.com/products/cortona. Our results showed that in regions where ameloblasts are polarized the capillaries are arranged in three distinct levels: (1) penetrating and leaving capillaries in relation to the outer enamel epithelium; (2) capillaries crossing and branching inside the stellate reticulum; and (3) capillaries branching and anastomosing profusely within the stratum intermedium, thereby forming an extensive capillary plexus intimately associated with the cells of the stratum intermedium. the existence of a conspicuous capillary plexus intermingled with cells of the stratum intermedium, as shown in our results, suggests that some molecules produced by cells of the stratum intermedium could be released into the capillary plexus and thereafter carried to the dental follicle.
Keywords capillaries
computer reconstruction
enamel organ
stellate reticulum
tooth germ
Language English
Date 2004-03-01
Published in Journal of Anatomy. Oxford: Blackwell Publishing Ltd, v. 204, n. 3, p. 191-195, 2004.
ISSN 0021-8782 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Ltd
Extent 191-195
Origin http://dx.doi.org/10.1111/j.0021-8782.2004.00268.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000189258200004
URI http://repositorio.unifesp.br/handle/11600/27637

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