Effect of estrogen on muscarinic acetylcholine receptor expression in rat myometrium

Effect of estrogen on muscarinic acetylcholine receptor expression in rat myometrium

Author Abdalla, FMF Google Scholar
Marostica, E. Google Scholar
Picarelli, Z. P. Google Scholar
Abreu, L. C. Google Scholar
Avellar, MCW Google Scholar
Porto, C. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Butantan
Abstract We report the effect of acute estrogen treatment in the expression of muscarinic acetylcholine receptors (mAChRs) in myometrium. Strips were obtained from rats in estrus (control) and treated with estrogen, 24 It before the experiments. Reverse transcriptase-polymerase chain reaction (RT-PCR) was performed and m2, m3 and m5 mAChR mRNA subtypes were detected in myometrium from both groups. [H-3]Quinuclidinyl benzilate ([(3)HQNB]) binding studies indicated that estrogen treatment did not change the affinity and density of mAChRs in myometrial membranes. Displacement curves of [(3)HQNB] with different mAChRs antagonists indicated a one-site fit for all antagonists tested. Comparison of pK(i) values indicated a significant correlation to M-2-mAChR subtype. Functional studies, however, showed that estrogen treatment increased myometrium sensitivity to carbachol and the calculated apparent affinity values were significantly correlated to M-3-mAChR. Furthermore, the pharmacological profile of the two populations of mAChR was not affected by estrogen. in conclusion, these results provide evidence for the presence of M-2- and M-3-mAChR, at the mRNA and protein level, in the rat myometrium and indicate that estrogen induces an increase in myometrial responsiveness to mAChR agonists. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
Keywords muscarinic acetylcholine receptors
myometrium
estrogen
carbachol
Language English
Date 2004-01-15
Published in Molecular and Cellular Endocrinology. Clare: Elsevier Sci Ireland Ltd, v. 213, n. 2, p. 139-148, 2004.
ISSN 0303-7207 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 139-148
Origin http://dx.doi.org/10.1016/j.mce.2003.10.040
Access rights Closed access
Type Article
Web of Science ID WOS:000189308800004
URI http://repositorio.unifesp.br/handle/11600/27582

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