Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea

Atypical enteropathogenic Escherichia coli strains: Phenotypic and genetic profiling reveals a strong association between enteroaggregative E-coli heat-stable enterotoxin and diarrhea

Author Dulguer, Michelle Vanzella Autor UNIFESP Google Scholar
Fabbricotti, Sandra Hilde Autor UNIFESP Google Scholar
Bando, S. Y. Google Scholar
Moreira, C. A. Google Scholar
Fagundes Neto, Ulysses Autor UNIFESP Google Scholar
Scaletsky, Isabel Cristina Affonso Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The virulence profiles of most atypical enteropathogenic Escherichia coli (EPEC) strains are unknown. A total of 118 typical and atypical strains of EPEC serotypes and non-EPEC serogroups isolated from children with or without acute diarrhea who were from different cities in Brazil were examined for virulence-associated markers and adherence to HEp-2 cells, and also had random amplified polymorphic DNA (RAPD) analysis performed. Atypical strains were identical to typical strains with regard to the virulence factors encoded on the locus of enterocyte effacement (LEE). in contrast with typical EPEC strains, none of the atypical strains reacted with the bfpA probe, and half of the strains hybridized with the perA probe. Most atypical strains presented Tir sequences that correlated with enteropathogenic or enterohemorrhagic E. coli (98%), had LEE inserted in either selC or pheU (88%), and presented a typeable intimin (52%). Eighteen new serotypes were found in the EPEC strains. Atypical and typical EPEC strains belonged to different RAPD clusters. Most atypical strains showed a localized-like adherence pattern (61.5%). of the non-LEE-encoded virulence factors, enteroaggregative E. coli heat-stable enterotoxin was noted most frequently (45%) and was significantly associated with diarrhea (P=.01). Thus, this virulence marker may be used as an additional tool for the diagnosis of truly atypical pathogenic strains.
Language English
Date 2003-12-01
Published in Journal of Infectious Diseases. Chicago: Univ Chicago Press, v. 188, n. 11, p. 1685-1694, 2003.
ISSN 0022-1899 (Sherpa/Romeo, impact factor)
Publisher Univ Chicago Press
Extent 1685-1694
Origin http://dx.doi.org/10.1086/379666
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000187493200011
URI http://repositorio.unifesp.br/handle/11600/27486

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