Human umbilical vein vasoconstriction induced by epinephrine acting alpha(1B)-adrenoceptor subtype

Human umbilical vein vasoconstriction induced by epinephrine acting alpha(1B)-adrenoceptor subtype

Author Errasti, A. E. Google Scholar
Avellar, MCW de Google Scholar
Daray, F. M. Google Scholar
Tramontano, J. Google Scholar
Luciani, L. I. Google Scholar
Bard, MJL Google Scholar
Marosica, E. Google Scholar
Rothlin, R. P. Google Scholar
Institution Univ Buenos Aires
Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVE: Our purpose was to determine the presence of alpha(1)-adrenoceptor messenger RNA subtypes and extend the pharmacologic characterization of alpha(1)-adrenoceptors involved in human umbilical vein (HUV) contraction.STUDY DESIGN: Cords (n = 124) from healthy patients after term vaginal or cesarean deliveries were used. the vein was carefully dissected out of cords and used for reverse transcription combined with polymerase chain reaction (RT-PCR) to amplify alpha(1)-adrenoceptor transcripts. in isolated organ baths, HUV rings were mounted and cumulative concentration-response curves were constructed either for epinephrine or the selective alpha(1A)-adrenoceptor agonist, A-61603. in other series of experiments, the effects of the selective alpha(1A)- and alpha(1B)-adrenoceptor antagonists (RS-100329 or B8805-033 or spiperone, AH11110A and cyclazosin, respectively) were evaluated to estimate its blocking potencies on epinephrine concentration-response curves.RESULTS: By means of RT-PCR technique alpha(1a)- and alpha(1b)-adrenoceptor transcripts were detected in the HUV. the blocking potency values of RS-100329 or B8805-033 against responses mediated by epinephrine were not consistent with the activation of an alpha(1A)-adrenoceptor population. Moreover, the low potency of the agonist A-61603 was not in accordance with an alpha(1A)-adrenoceptor interaction. On the other hand, the antagonist potencies of spiperone, AH11110A and cyclazosin were in agreement with an interaction on alpha(1B)-adrenoceptor subtype.CONCLUSION: Although alpha(1a)- and alpha(1b)-adrenoceptor messenger RNAs are detected in the HUV, only alpha(1B)-adrenoceptors are involved in epinephrine vasoconstrictor action.
Keywords human umbilical vein
fetal distress
Language English
Date 2003-11-01
Published in American Journal of Obstetrics and Gynecology. St Louis: Mosby, Inc, v. 189, n. 5, p. 1472-1480, 2003.
ISSN 0002-9378 (Sherpa/Romeo, impact factor)
Publisher Mosby, Inc
Extent 1472-1480
Access rights Closed access
Type Article
Web of Science ID WOS:000186979500046

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