Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4

Immunolocalization of Leishmania (Viannia) braziliensis membrane antigens recognized by mAbs SST-2, SST-3, and SST-4

Author Silveira, TGV Google Scholar
Takahashi, H. K. Google Scholar
Straus, A. H. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The immunolocalization of Leishmania (Viannia) braziliensis stage-specific antigens recognized by mAbs was analysed by transmission electron microscopy. the antigen recognized by mAb SST-2 was present at the surface of promastigotes, including the flagellum and flagellar pocket. the reactivity of SST-2 with isolates of different serodemes showed a pronounced microheterogencity in terms of the number of reactive bands within the low molecular weight range from 24 to 33 kDa. the 180 kDa glycoprotein recognized by mAb SST-3 was present only in the flagellar membrane. SST-3 also recognized multiple discrete bands from 160 to 200 kDa, as observed in several serodemes. in contrast, mAb SST-4, which recognizes a 98 kDa antigen, showed weak labelling on the promastigote surface by transmission electron microscopy and indirect immunofluorescence. Based on Western blotting, indirect immunofluorescence, and solid-phase radioimmunoassay, the antigens recognized by mAbs SST-2, SST-3 and SST-4 were present in all L. (V.) braziliensis analysed, from 7 different serodemes.
Keywords Leishmania (Viannia) braziliensis
monoclonal antibody
specific antigens
flagellum
Language English
Date 2003-11-01
Published in Parasitology. New York: Cambridge Univ Press, v. 127, p. 449-456, 2003.
ISSN 0031-1820 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 449-456
Origin http://dx.doi.org/10.1017/S0031182003003986
Access rights Closed access
Type Article
Web of Science ID WOS:000187030600005
URI http://repositorio.unifesp.br/handle/11600/27471

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